Enzymes

k1
k1

k2

Vmax [S]
v
Km [S]

20

5
4

-Km, Vmax

10

v, mol/min

v, mol/min

15

5
0

Km

-5
-10
-50

0.5Vmax

-30

-10
10
[S], mM

30

50

10

20
30
[S], mM

40

Eric Niederhoffer
SIU-SOM

50

Competitive Inhibition
EI

Competitive
v
5

Vmax [S]
K m [S]

2.5

+CI
0.5V
max

Km

Kmapp

-1/K

Km
0

10

20
30
[S], mM

40

50

app
m

2
1/v, /mol/min
/mol/min
1/v,

[I]
1

Kic

0.5Vmax

CI

No I

4
v, mol/min
mol/min
v,

Vmax [S]
Km [S]

Kic
S+E
ES
E+P
E
+
I
1 Km 1
1
1 K m 1
1

v Vmax [S] Vmax

v Vmax [S] Vmax

-1/Km
-1/K
m

+CI

Kmapp/Vmax

1.5
1
0.5

-0.6 -0.4 -0.2

K /Vmax
K m
/V
m max

No I

1/Vmax
1/Vmax

0 0.2 0.4 0.6 0.8

1/[S], /mM

Uncompetitive Inhibition
Uncompetitive
(catalytic)
v

Vmax [S]
Km [S]

UCI

S+E

1 Km 1
1

v Vmax [S] Vmax

2.5

0.5Vmax
+ UC I
0.5V
max

Km

2
1

Km
0

10

0.5Vmax

[I]
' 1

Kiu

app

Km

20
30
[S], mM
[S].

-1/Km
40

50

1/v, /mol/min

v, mol/min

Vmax [S]
Km ' [S]

No I

-1/K
-1/Kmm
app

1.5

ESI
Kiu
ES
E+P
+
I
1 Km 1
'

v Vmax [S] Vmax

1/Vmaxapp
Kmapp/Vmaxapp

1
0.5

-0.6 -0.4 -0.2

1/Vmax

Km/Vmax
Km/Vmax + UC I
No I

1/Vmax
0 0.2 0.4 0.6 0.8
1/[S]. /mM
1/[S],

Noncompetitive Inhibition

v
55

v, mol/min

Vmax [S]
Vmax [S]
v
Km [S]
K m ' [S]
No I

44

0.5Vmax

33

0.5V
+ NCImax

0.5Vmax

22
11
00

NCI

Km
00

10

Kic
S+E
+
NCI
I
1 Km 1
1

v Vmax [S] Vmax

2.5

[I]
1

Kic

[I]
' 1

Kiu

-1/K
-1/K
m
m

Km

20
30
[S],
[S], mM
mM

1/v, /mol/min
/mol/min
1/v,

Noncompetitive
(mixed-type)

EI

40
40

50
50

1.5

ESI
Kiu
ES
E+P
+
I
1 K m 1
'

v Vmax [S] Vmax

1/Vmaxapp
Km/Vmaxapp
+ NC I

1
0.5

-0.6 -0.4 -0.2

1/Vmax

KmK/Vmax
m/Vmax

1/Vmax
0 0.2 0.4 0.6 0.8
1/[S], /mM

No I

Enzyme Inhibition Summary

V [S]
Vmax [S]
v max
v
Km [S]
K m ' [S]
No inhibitor

v, mol/min

+ UC I

3
2

Kapp m
Km
Km

1
0

[I]
1

Kic

+CI

10

0.5Vmax

40

-1/Km

-1/K app
m

0.5Vmaxapp
app
Kappm 0.5V
max
50

1 K m 1
'

v Vmax [S] Vmax

Kmapp/Vmax

+CI

app
2 Km/Vmax

[I]
' 1

Kiu

+ NC I

20
30
[S], mM

2.5

1/v, /mol/min

1 Km 1
1

v Vmax [S] Vmax

-1/Km
-1/Km

+ NC I

app
1/Vmax
1/Vmaxapp

+ UC I
No I

0.5

-0.6 -0.4 -0.2

Neat or what?

K mapp /Vmaxapp

1.5

K /V
1/Vmax m max
0 0.2 0.4 0.6 0.8
1/[S], /mM

Review Questions

What is the function of enzymes?

What do enzyme names mean?
What are the types of enzyme
inhibition (functional and
graphical features)?