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    Microscopes Short

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    Microscopes allow us to see very small things like cells that are too small to be seen with the naked eye. The compound light microscope uses two lenses, an objective lens and an ocular lens, to magnify a specimen placed between them. Light passes through the specimen and lenses and produces an enlarged virtual image that is upside down and backwards. The total magnification is calculated by multiplying the magnifying powers of the objective and ocular lenses. Specimens are often stained with dyes to make cell structures more visible. Focusing involves using coarse and fine adjustments on the lenses. Dissecting and electron microscopes show larger or smaller magnified views than compound light microscopes.

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    Microscopes Short

    Uploaded by

    api-299996815
    134 views13 pages
    Microscopes allow us to see very small things like cells that are too small to be seen with the naked eye. The compound light microscope uses two lenses, an objective lens and an ocular lens, to magnify a specimen placed between them. Light passes through the specimen and lenses and produces an enlarged virtual image that is upside down and backwards. The total magnification is calculated by multiplying the magnifying powers of the objective and ocular lenses. Specimens are often stained with dyes to make cell structures more visible. Focusing involves using coarse and fine adjustments on the lenses. Dissecting and electron microscopes show larger or smaller magnified views than compound light microscopes.

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    Microscopes allow us to see very small things like cells that are too small to be seen with the naked eye. The compound light microscope uses two lenses, an objective lens and an ocular lens, to magnify a specimen placed between them. Light passes through the specimen and lenses and produces an enlarged virtual image that is upside down and backwards. The total magnification is calculated by multiplying the magnifying powers of the objective and ocular lenses. Specimens are often stained with dyes to make cell structures more visible. Focusing involves using coarse and fine adjustments on the lenses. Dissecting and electron microscopes show larger or smaller magnified views than compound light microscopes.

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    © All Rights Reserved
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    134 views13 pages

    Microscopes Short

    Uploaded by

    api-299996815
    Microscopes allow us to see very small things like cells that are too small to be seen with the naked eye. The compound light microscope uses two lenses, an objective lens and an ocular lens, to magnify a specimen placed between them. Light passes through the specimen and lenses and produces an enlarged virtual image that is upside down and backwards. The total magnification is calculated by multiplying the magnifying powers of the objective and ocular lenses. Specimens are often stained with dyes to make cell structures more visible. Focusing involves using coarse and fine adjustments on the lenses. Dissecting and electron microscopes show larger or smaller magnified views than compound light microscopes.

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    © All Rights Reserved
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    Download as pptx, pdf, or txt
    of 13

    Aim: How do we observe the tiny

    parts of the world around us?

    Microscopes
    Allow us to see very small things like
    cells
    Things that we cant see with the
    naked/unaided eye

    The Compound Light Microscope


    Most commonly
    used for cell study
    Specimen (thing
    being viewed) has
    to be thin enough
    that light can pass
    through it easily
    2 lenses
    Objective lens
    Ocular/eyepiece
    lens

    How does light pass through the


    microscope?
    Magnifies

    Light

    Specimen

    Objective
    Lens

    Magnifies

    Ocular/
    Eyepiece
    Lens

    Your
    Eye!

    The final image


    The final image your eye sees
    appears bigger, upside down, and
    backwards. (180 degree rotation)

    Total Magnification- how much bigger the object you


    see through the microscope is than the actual object

    Total
    Magnification

    Magnifying
    =
    Power of
    Objective Lens

    Magnifying
    Power of Ocular
    Lens/Eyepiece

    Focusing the Compound Light


    Microscope
    1. Start with the lowest power objective (10x)
    2. Focus using the coarse adjustment (big knob)
    3. Switch to the next higher power objective
    ***When you are on the highest power objective, ONLY
    USE THE FINE ADJUSTMENT!!!! Otherwise you could
    break the slide!***
    As you switch from low to high power, the field of view becomes
    darker. To deal with this, the diaphragm needs to be opened to
    allow in more light.

    4. As you switch from low to high power the field of


    view becomes smaller.

    Staining
    Many parts of the cell are almost
    colorless and are hard to distinguish
    from other cell parts
    We add stain to color cell parts so we
    can see them better under the
    microscope
    Common stains:
    Iodine
    Methylene blue

    Adding Stain (w/o removing


    coverslip)

    Making a Slide (a wet-mount)

    Dissecting Microscope
    Allows us to study larger
    3D specimens
    Ex. Insects
    Things that CAN be
    seen with the naked eye
    2 eyepieces and 2
    objective lenses
    Helpful in performing
    dissections

    Electron Microscope
    Uses a beam of electrons
    instead of light
    Focused by magnets
    instead of lenses
    Disadvantage: can only
    use to view dead
    specimens
    Advantage: very high
    magnification allows us to
    see internal structure of
    cells

    Cool electron microscope images


    http://www.topdesignmag.com
    /25-amazing-electron-microscope-images/

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