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Slide Biop 4-Media
Slide Biop 4-Media
Slide Biop 4-Media
Nutritional Requirements
The facts
Required Medium
.considering
availability
of nutrients required by
microorganisms: energy, grow,
production
fermentation conditions: substrate pH,
MC, incubation temperature
stage of growth of micro-organisms
presence of other competing microorganisms
Preparation & downstream process
Natural or formulated-defined
media?
Natural Media:
Cheaper
High variation/batch
Unpredictable
product/biomass
More complex in
down stream process
Difficult to detect
small yield
improvement
Formulated-MEDIA
Easily reproduce
Specific to the strain
More expensive
More predictable
result
More simple process
cheaper
Easier to detect
process improvement
MEDIA FORMULATION
Satisfy
C& energy+N+O2+others
biomass+P+CO2+H2O+heat
product
Organism
Energy
Water
Major comp. require in fermentation Use
for solvent within the cell
Different in percentage
it has some unusual properties ionizes into
acid and base, and has a propensity for
hydrogen bonding
May need treatments
Consider: pH, salts, mineral, effluent
contaminant
Carbon
Trace elements
In mM level : N, P, S, K and Mg
In M level : Fe, Na, Ca, Cu, Co, Zn, Mn etc.
Supplied during cell cultivation
Various functions: serve in coenzyme, catalyse
reactions, vitamin synthesis, cell transport
very low levels; sometimes supplied from quantities
occurring in water or from leachates from
equipment
Primary metabolite production is usually not very
sensitive to trace element concentration, but not
for secondary metabolite production
Growth Factors