Scribd Logo
Upload

Welcome to Scribd!

  • FALLSEM2016-17 BIF311 TH 1595 13-SEP-2016 RM001 Prediction of Protein 3D Structure

    Uploaded by

    Bhagvad Jaideep
    8 views27 pages
    This document discusses experimental and theoretical methods for determining protein structures. Experimental methods like X-ray crystallography provide the most accurate structures but require crystals and are expensive, while NMR can be used in vivo but is limited to small proteins. Cryo-electron microscopy provides lower resolution structures. Theoretical prediction methods are important because sequencing outpaces experimental structure determination, leaving more sequences than structures known. Predicted structures can be used for drug development, predicting protein function, and interpreting experimental data.

    Original Description:

    BIF

    Copyright

    © © All Rights Reserved

    Available Formats

    PPT, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    This document discusses experimental and theoretical methods for determining protein structures. Experimental methods like X-ray crystallography provide the most accurate structures but require crystals and are expensive, while NMR can be used in vivo but is limited to small proteins. Cryo-electron microscopy provides lower resolution structures. Theoretical prediction methods are important because sequencing outpaces experimental structure determination, leaving more sequences than structures known. Predicted structures can be used for drug development, predicting protein function, and interpreting experimental data.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    8 views27 pages

    FALLSEM2016-17 BIF311 TH 1595 13-SEP-2016 RM001 Prediction of Protein 3D Structure

    Uploaded by

    Bhagvad Jaideep
    This document discusses experimental and theoretical methods for determining protein structures. Experimental methods like X-ray crystallography provide the most accurate structures but require crystals and are expensive, while NMR can be used in vivo but is limited to small proteins. Cryo-electron microscopy provides lower resolution structures. Theoretical prediction methods are important because sequencing outpaces experimental structure determination, leaving more sequences than structures known. Predicted structures can be used for drug development, predicting protein function, and interpreting experimental data.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    of 27

    Theoretical structure prediction

    methods

    Experimental Protein Structure


    Determination
    X-ray crystallography

    most accurate
    in vitro
    needs crystals
    ~$100-200K per structure

    NMR

    fairly accurate
    in vivo
    no need for crystals
    limited to very small proteins

    Cryo-electron-microscopy
    imaging technology
    low resolution

    Why not just solve the structures


    experimentally?
    Some structures are very difficult to solve
    experimentally
    Sometimes many labs work for decades to solve
    the structure of one protein
    Sequence determination far outpaces
    experimental structure determination
    We already have far more sequences than
    experimental structures, and this gap will likely
    grow

    Why not just solve the structures


    experimentally

    How are predicted structures


    used?
    Drug development
    Computational screening of candidate drug
    compounds
    Figuring out how to optimize a promising candidate
    compound
    Figuring out which binding site to target
    Predicting the function of a new protein
    Explaining how a protein functions, and how one might alter
    its function (e.g., with a drug)
    Interpreting experimental data
    For example, a computationally predicted approximate structure
    can help in determining an
    accurate structure experimentally.

    You might also like