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    Electrophoresis: Analisa Hasil Pertanian

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    Umam Gooners Smilikity
    Gel electrophoresis is a technique that separates DNA and RNA molecules by size, shape, and topological properties. A gel matrix like agarose or polyacrylamide supports the movement of macromolecules through its porous structure under an electric field toward the positive pole. Linear DNA molecules separate based on size, while circular DNA topology also affects mobility, with supercoiled DNA moving fastest and relaxed or nicked DNA moving slowest. The concentration of DNA in a sample can be estimated based on its position relative to a marker of a known concentration on the gel.

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    Electrophoresis: Analisa Hasil Pertanian

    Uploaded by

    Umam Gooners Smilikity
    36 views8 pages
    Gel electrophoresis is a technique that separates DNA and RNA molecules by size, shape, and topological properties. A gel matrix like agarose or polyacrylamide supports the movement of macromolecules through its porous structure under an electric field toward the positive pole. Linear DNA molecules separate based on size, while circular DNA topology also affects mobility, with supercoiled DNA moving fastest and relaxed or nicked DNA moving slowest. The concentration of DNA in a sample can be estimated based on its position relative to a marker of a known concentration on the gel.

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    Electrophoresis

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    Electrophoresis

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    Gel electrophoresis is a technique that separates DNA and RNA molecules by size, shape, and topological properties. A gel matrix like agarose or polyacrylamide supports the movement of macromolecules through its porous structure under an electric field toward the positive pole. Linear DNA molecules separate based on size, while circular DNA topology also affects mobility, with supercoiled DNA moving fastest and relaxed or nicked DNA moving slowest. The concentration of DNA in a sample can be estimated based on its position relative to a marker of a known concentration on the gel.

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    © All Rights Reserved
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    36 views8 pages

    Electrophoresis: Analisa Hasil Pertanian

    Uploaded by

    Umam Gooners Smilikity
    Gel electrophoresis is a technique that separates DNA and RNA molecules by size, shape, and topological properties. A gel matrix like agarose or polyacrylamide supports the movement of macromolecules through its porous structure under an electric field toward the positive pole. Linear DNA molecules separate based on size, while circular DNA topology also affects mobility, with supercoiled DNA moving fastest and relaxed or nicked DNA moving slowest. The concentration of DNA in a sample can be estimated based on its position relative to a marker of a known concentration on the gel.

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    ELECTROPHORESIS

    Analisa Hasil Pertanian

    ectrophoresis

    1. Gel electrophoresis separates


    DNA and RNA molecules
    according to size, shape and
    topological properties
    Gel matrix is an inserted, jello-like
    porous material that support and
    allows macromolecules to move
    through. Agarose and polyacrylamide
    are two different gel matrices

    ectrophoresis
    DNA and RNA molecules are
    negatively charged, thus move in the
    gel matrix toward the positive pole (+)
    Linear DNA molecules are separated
    according to size
    The mobility of circular DNA
    molecules is affected by their
    topological structures. The mobility
    of the same molecular weight DNA
    molecule with different shapes is:
    supercoiled> linear> nicked or relaxed

    Elektroforesis

    Fig 20-1: DNA separation by gel electrophoresis

    large

    moderate

    small

    After electr

    Konsentrasi DNA

    Konsentrasi DNA=
    pita yg mirip dgn marker/jmlh bp marker (55675) x
    500 ng

    EtBr

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