Encapsulation

of
Curcumin
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Into
Layered Double Hydroxides
Supun
Samindra
Dr. N.S.
Kottegoda
Institute
of Chemistry,
Ceylon
College of Chemical
1

INTRODUCTION
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Turmeric (Curcuma longa) is a medicinal plant extensively

used in Ayurveda and household remedy for various
diseases
Curcumin is one of active components in turmeric
Curcumin is the phyto-chemical that gives a yellow
colour to turmeric and responsible for most of the
therapeutic effects including antimicrobial and
wound healing activity
Poor thermal stability of curcumin restricts to
remain in few number of therapeutic applications
Pure curcumin was extracted by using silica gel
column chromatography
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INTRODUCTION

cont

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Layered Double Hydroxides (LDHs) are the most well-known

anionic clays and derived from the structure of mineral
brucite
LDH consists of a hexagonal close packing of hydroxyl ions
in which alternate layers of octahedral sites are occupied by
Mg2+ and Al3+ ions

The solubility of LDHs is highly

pH-dependent
Physicochemical properties of LDH for
application as anion adsorbents, medicinal
stabilizers, ion exchangers, catalyst,
ion conductors and hybrid systems

HOST-GUESTCHEMISTRY
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Host-guest
chemistrydescribescomplexesthat
are
composed of two or moremoleculesorionsthat are held
together in unique structural relationships by forces other
than those of fullcovalent bonds
Intercalation reaction between the host (LDH)
and guest (Curcumin) compounds provide the
opportunity to develop various hybrid compounds
for pharmaceutical use
A huge variety of anions can be incorporated into
the interlayer region of these hosts using a range of
methods
Co-precipitation method is one of the methods
commonly used for preparation of
LDH intercalated
materials
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INTERCALATION OF LDH IN
CURCUMIN MEDIUM
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The Mg-Al solution was added

drop wise to a solution
containing Curcumin under
vigorous stirring conditions at
60oC

Preparation of Mg-Al
solution
(molar ratio 2:1) using
Mg(NO3)2
and Al(NO3)3

Washed
with
distilled
water
Dried at 90
o
C

Filtere
d
LDHCurcumin
hybrid
material

During the addition

period, the pH of the
solution was
maintained at 9 by
adding NaOH

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Characterizati
on of LDHcurcumin
Nanocomposite
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PXRD ANALYSIS
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Figure: PXRD spectra of pure LDH

[BLACK] and LDH-curcumin7

FT-IR ANALYSIS
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Figure: FT-IR spectra of (a) Pure LDH (b) Pure

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curcumin and

UV-VISIBLE SPECTROSCOPY
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Figure: UV-Visible spectrum of LDH9

curcumin

UV-VISIBLE SPECTROSCOPY
Table:hcshhsdhs
The sample and their respective
max
Sample
max /nm
Pure LDH

301.00

Curcumin

425.00

LDH- curcuimn

459.00

LDH-curcumin composites absorb light in the 400 to

500 nm region
These light absorbing groups are referred to as
chromophores
LDH-curcumin hybrid system is more stable than pure
curcumin
Figure: Curcumin exhibits keto-enol
tautomerization

10

cont

THERMOGRAVIMETRIC
ANALYSIS(TGA)
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Figure: TGA thermogram of LDH11

Curcumin

THERMO GRAVIMETRIC
ANALYSIS(TGA)cont
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Figure: The sample and their respective
water loss
Sample
Water loss / %
Pure LDH

55.78

LDH- curcumin

17.62

Interlayer of LDH consists of NO3- ions and water

molecules.
In LDH-curcumin nano-composite, the interlayer spacing
consists of NO3- ions, water molecules and curcumin
Therefore, curcumin-LDH consists of much lower water
content compared to pure LDH because the curcumin
molecules replace water molecules and curcumin
molecule accommodates at a larger spacing compared to
water molecule
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NNING ELECTRON MICROSCOPY (

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[A]

[B]

Figure: SEM images of [a] Pure Mg-Al-LDH

[b] LDH-curcumin
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UV DEGRADATION OF CURCUMIN
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To measure the effects of UV radiation, expose the
extracted curcumin sample into continuous UV light source
then the absorbance was monitored throughout the test
period

Time/hr

Absorbance

Percentage
reduction/ %

0
0.5
1.0
1.5
2.0

3.099
3.087
3.064
3.047
3.016

0
0.38
1.13
1.68
2.68

Table : Absorbance vs. time of UV-degradation of

curcuimn and
corresponding percentage reductions
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UV DEGRADATION OF CURCUMIN
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Figure: Degradation products of

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curcumin

UV DEGRADATION OF CURCUMIN
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Figure: The graph of absorbance vs. time of UVdegradation of curcumin

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Kinetic Study

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KINETIC STUDY OF THE UVRELEASE OF LDHCURCUMIN

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The Zero order drug releasing equation of
curcumin at pH 2 is

Where:
Q0 = Initial amount of drug
Qt = Amount of drug release at time t
K0 = Zero order release constant
t = Time in hours
Rearranging of the above equation into:
Qt / Q0 = 1 + K0 t / Q0
Therefore,

A0: Initial absorbance of drug

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At: Absorbance of drug release at timet

KINETIC STUDY OF THE

UV-RELEASE OF LDHhcshhsdhs
CURCUMIN cont

Figure: (At / A0) vs. time for UV-release of19curcumin

at pH 2

CONCLUSION
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PXRD, FT-IR, UV-Vis spectroscopy, SEM and TGA

results
confirm
that
the
curcumin
was
successfully intercalated to LDH
Curcumin exhibits keto-enol tautomerization at
higher pHs, as a result overall charge of curcumin
becomes negative and the hydrophilic nature of
OH allows to strong interactions with LDH
At lower pHs, curcumin intercalated LDH releases
the curcumin more linearly due to the
destabilization of interactions between LDH and
curcumin
The kinetic study suggests the use of curcumin
intercalated LDH at low pH hence this concept
can be incorporate into a drug carrier or wound
dresser in plaster industry
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ACKNOWLEDGEMENT
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Dr.Nilwala Kottegoda
Prof. J.N.O. Fernando
Prof. S. Sotheeswaran
Dr. U.S.K. Weliwegamage
All the academic and non-academic members of
SLINTEC and Institute of Chemistry ,Ceylon
Mr. R. Hasantha
Miss Princy Selvaraj
Mr.Sahan Perera
Mr. Dilshan Gunasinghe
My mother, family members and my friends
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Thank
You
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