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Methods of Examination Mycology
Methods of Examination Mycology
Methods of Examination Mycology
EXAMINATION
mycology
Temporary Mount
KOH 10-20%: used for specimens
with distracting tissue debris and
cells(vaginal secretions, nails)
Permanent Mounts
Gram Stain: for detection of yeast
(+)
Fontana-Masson Stain:
demonstration of melanin or melanin
like substances in the lightly
pigmented agents of
phaeohypomycosis
Recommended incubation
time:
7 days yeast in mouth, throat and
vagina
21 days fungal pathogens in tissues
and sterile body fluids other than
blood
Up to 28 days respiratory, BM,
blood specimens and specimens in
which a dimorphic fungi is suspected
Special Tests
Hair Baiting Test differentiates T.
mentagophytes (+) from T. rubrum
(-)
Commercially Available
Systems
Sensititre Yeast One Test Panel
microtitre broth dilution method,
consist to a disposable microtitre
plate, results are expressed in MIC,
excellent shelf life, for molds too.