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Neisseria Gonorrhoeae
Neisseria Gonorrhoeae
Source of infection:
1. Asymptomatic carriers
2. Patients
Mode of infection:
1. Venereal infection (sexual contact)
2. Nonvenereal infection
Antigenic structure & virulence factors:
2. Lipooligosaccharide: Endotoxic.
Specimens collected:
A) In men:
a) Acute infection- Urethral discharge
b) Chronic infection-
i) Morning drop
ii) Discharge collected after prostatic massage
iii) Centrifuged deposit of urine
B) In women:
i) Urethral discharge
ii) Cervical swabs
C) In both the sexes: Blood, CSF, synovial fluid,
throat swab, rectal swab & material from skin
rashes.
Transport: If there is delay in processing than the
specimens should be sent in Stuarts medium.
Methods of examination:
A) Direct microscopy:
1. Gram staining:
Smear provides a
presumptive
evidence
of gonorrhea in men.
Gram negative
diplococci are found.
But it is unreliable
in women.
2. Immunofluorescence:
B) Culture:
Media used:
Colony morphology:
Grams smear:
Reveals Gram negative
cocci in pairs with
adjacent sides concave.
Biochemical reactions:
C) Serology:
Precipitation,
Passive agglutination,
Immunofluorescence,
Radioimmunoassay.
TREATMENT:
Tracing of contacts,
Health education,
General measures,