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    Part 16 Lab

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    Novia Adriani
    There are several methods for determining the type of microbe in a laboratory examination, including urine and feces tests. Urine tests examine characteristics like specific gravity, proteins, reductions, and pigments. Feces tests examine similar factors. Bacteriological examinations analyze samples from sources like sputum, nasal/throat swabs, vaginal swabs, wounds, urine, and feces. Single and double staining techniques like methyl blue/red, Ziehl-Nielsen, and Gram staining are used to identify bacteria under a microscope based on color and cellular morphology. Proper sample collection and preparation are important for accurate results.

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    Part 16 Lab

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    Novia Adriani
    5 views15 pages
    There are several methods for determining the type of microbe in a laboratory examination, including urine and feces tests. Urine tests examine characteristics like specific gravity, proteins, reductions, and pigments. Feces tests examine similar factors. Bacteriological examinations analyze samples from sources like sputum, nasal/throat swabs, vaginal swabs, wounds, urine, and feces. Single and double staining techniques like methyl blue/red, Ziehl-Nielsen, and Gram staining are used to identify bacteria under a microscope based on color and cellular morphology. Proper sample collection and preparation are important for accurate results.

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    part 16 lab

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    There are several methods for determining the type of microbe in a laboratory examination, including urine and feces tests. Urine tests examine characteristics like specific gravity, proteins, reductions, and pigments. Feces tests examine similar factors. Bacteriological examinations analyze samples from sources like sputum, nasal/throat swabs, vaginal swabs, wounds, urine, and feces. Single and double staining techniques like methyl blue/red, Ziehl-Nielsen, and Gram staining are used to identify bacteria under a microscope based on color and cellular morphology. Proper sample collection and preparation are important for accurate results.

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    Download as ppt, pdf, or txt
    5 views15 pages

    Part 16 Lab

    Uploaded by

    Novia Adriani
    There are several methods for determining the type of microbe in a laboratory examination, including urine and feces tests. Urine tests examine characteristics like specific gravity, proteins, reductions, and pigments. Feces tests examine similar factors. Bacteriological examinations analyze samples from sources like sputum, nasal/throat swabs, vaginal swabs, wounds, urine, and feces. Single and double staining techniques like methyl blue/red, Ziehl-Nielsen, and Gram staining are used to identify bacteria under a microscope based on color and cellular morphology. Proper sample collection and preparation are important for accurate results.

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    of 15

    BY : Dewi Oktavia,S.

    KM
    1. Cara menentukan jenis suatu
    mikroba :
    2. Pemeriksaan
    laboratorium
    1). Pemeriksaan Urin
    2). Pemeriksaan Feses
    Metode
    1. Berat jenis dg menggunakan urometer (urine
    meter)
    2. Protein dg :
    Percobaan masak
    Exton
    Bang
    Esbach
    3. Reduksi dg :
    Fehling
    Benedict
    4. Urobilin dg : Schlesinger
    5. Bilirubin dg modefikasi Fouchet(Bapar
    test)
    6. Badan-badan keton dg :
    Rotharg
    gerhard
    3. Pemeriksaan Bakteriologi
    Bahan2 :
    1) Sputum (TBC)

    2) Apus hidung

    3) Apus tenggorokan (Difteria)

    4) Apus Vagina (GO)

    5) Luka, abses

    6) Urin, feses
    Pengocokan :
    1. Pengocokan single dg methyl blue atau
    methyl red.
    2. Pengocokan double dg Ziehl Nielson atau
    Gramhielser
    Cara Ziehl Nielsen
    Pengocokan Gram
    Pengocokan Neisser
    Pengocokan single dg methyl blue atau
    methyl red.
    Bhn yg dperiksa dioleskan pd objek glass
    >>> dipanaskan >>> beri warna
    methyl blue/methyl red >>>objek glass
    dicuci & dikeringkan >>> mikroskop.
    Hasil : jika ada methyl red >>> bakteri
    merah
    jika ada methyl blue >>> bakteri biru
    Pengocokan double dg Ziehl Nielson
    atau Gramhielser
    Cara Ziehl Nielsen
    Bakteri tahan panas (M.tuberculosa)
    Sputum diencekan dg NaCl 0,9%
    Sputum diletakkan pd objek glass dihimpit dg dek gelas
    (agar rata) kedua kaca dpisahkan lalu dikeringkan
    Beri karbol fuchsin dan panaskan hingga menguap
    slma 3 mnt, lalu cuci
    Beri alkohol (3-10 mnt) segera dicuci lg dg air
    Beri methil blue (1 mnt)
    Cuci lg lalu keringkan>>> mikroskop
    Hasil : bakteri brbentuk batang bewarna merah
    Pengocokan Gram
    Smw bakteri
    Letakkan Nanah atau sekret vagina pd objek glass

    Beri karbol violet (3 mnt)

    Beri cairan logol 30-60 detik jgn dcuci

    Beri ethyl alkohol 96% slama 45 mnt lalu dicuci dg air

    Baru beri larutan Fuchsin (biarkan 1 mnt)

    Dicuci dg air akuades dan keringkan.

    hasil : bakteri gram positif bwrna ungu, gram negatif

    wrna merah.
    GO >bakteri bentuk biji kopi >>gram negatif
    Pengocokan Neisser
    Khusus pykt Difteria
    Apus tenggorokan dioleskan pd objek

    glass>>keringkan
    Beri dg Neisser (1-2 mnt) lalu keringkan

    Mikroskop

    Hasil : kuman berbentuk batang


    ditengah bewarna kuning dan katupnya
    bewrna bintik hitam.
    Agar hasil memuaskan :
    Biakan (kultur) >>> test sensitivitas
    resinstensi (uji kepekaan obat)

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