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Bio Reactor
Bio Reactor
Bio Reactor
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Bioreactor Scale-up
Scale-up:
- Geometric similarity of the physical boundaries:
- same type of reactor
- all linear dimensions of the model must be related to the
corresponding dimensions of the prototype by a constant scale-up
factor.
i.e. Keep the ratio of the height H to diameter Dt (tank) same in
the model and prototype.
Normally H/Dt is 2~3.
Hp Dp
Scale-up factor: ...
Hm Dm
Ratio of surface to volume decreases during scale-up.
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Bioreactor Scale-up
- Dynamic similarity of the flow fields
To achieve dynamic similarity in a stirred-tank reactor, scale-up can
be based on the following criteria in addition to geometric similar
boundaries.
constant power input per volume: P0/V or
constant liquid circulation rate inside the reactor: Q/V
(pumping rate of impeller per unit volume)
constant impeller tip speed (shear): NDi
constant Reynolds number: NDi2/
V: working volume
P0: energy input (W); N: impeller speed (rpm); : density
(kg/m3)
Di: impeller diameter (m),30-40% of the diameter of the tank
(Dt)
: viscosity (kg/ms);
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Bioreactor Scale-up
Relating the above criteria to impeller diameter
Di and
speed N:
P N 3 Di 5 ; V Di 3 ; Q NDi 3
(Perrys Chemical Engineers Handbook, 7th Ed. Page.18-11)
Design Criteria
5 3 2
constant power input per volume : P / V N 3 Di / Di N 3 Di
3 3
constant liquid circulatio n rate : Q/V NDi / Di N ;
constant impeller t ip speed NDi
2
constant Reynolds number Re NDi
Since Di Dt , the above relationsh ip is also valid for Dt . 20
Bioreactor Scale-up
In scale-up of a stirred-tank reactor, the design
calculations are as follows:
- Determine the scale-up factor Dp/Dm
- Based on the geometric similarity, calculate the dimensions of the
prototype (height H and diameter Dt of tanks, impeller diameter Di if
possible) by multiplying that of the model with the scale-up factor.
- Select criterion related to dynamic properties and keep it constant in
both the model and the prototype.
- Determine the parameters such as impeller speed or diameter for the
scale-up reactor.
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Bioreactor Scale down
To provide an experimental system at a
smaller scale that duplicates the environment
that exists at the larger scale.
- Mimic the production facilities at a smaller scale
- Parameters can be tested more quickly and inexpensively than at the
production scale.
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