Báo Cáo TH C Hành Hóa Hưu Cơ Nhóm 1

KHOA: CNG NGH HA HC
Cho mng C v cc bn n vi bi
thuyt trnh ca nhm 1
Mn: Thc Hnh Ha Hu C
GVHD: Ths. V Uyn Vy
Sinh vin : Trn Vn Sng
: Mai Tun Anh
: Trn Hong Sn
NI DUNG BI THC HNH
1
Tnh baz ca amin
2 6
Phn ng ca 7
Phn ng
Phn ng Biure
amin bc 1
Xantoprotein
3 4 5
Phn ng ca Phn ng ca Phn ng ca
anilin vi HNO2 amin bc 2 amin bc 3
www.trungtamtinhoc.edu.vn
NI DUNG BI THC HNH
TN 8: Phn ng vi ninhidrin
TN 9: Phn ng oxi ha monosaccarit bng
phc cht ca bc vi ammoniac
TN 10: Phn ng oxi ha monosaccarit
bng ng (II) hydroxit
TN 11: Phn ng oxi ha monosaccarit bng thuc th Feling
TN 12: Phn ng oxi ha monosacarit bng nc brom
TN 13: Phn ng thy phn saccararozo, tinh bt
trong mi trng axit
TN 14: Phn ng ca tinh bt vi iod
KT QU TH NGHIM
Th nghim 1: Tnh baz ca amin
0,2 ml
0,2 ml CH3NH2 2 git PP CH3NH2
2 git PP
Lc u
Dung dch
Quan st
chuyn sang
hin tng
mu hng
KT QU TH NGHIM
Phng trnh phn ng
HO OH O O
C 2CH3NH2 C
O O 2CH3NH3
CO CO
PP
Gii thch hin tng
Metylamin c tnh bazo nn khi cho

phenolphatalein vo th dung dch chuyn sang
mu hng
KT QU TH NGHIM
Th nghim 2: Phn ng ca amin bc 1
0,5 ml
CH3NH2 2ml HCl
2ml HCl 10%
Lm lnh
(bng nc )
Dd NaNO2 20%
Quan st
hin tng
KT QU TH NGHIM
Amin bc 1 (metylamin-CH3NH2) tc dng vi
HNO2(NaNO2+HCl) to ra mui diazonium.
CH 3 NH 2 HCl NaNO2 CH 3 N N Cl H 2O
Mui diazonium trong mi trng c to = 0-5oC s

phn hy thnh metanol v kh N2 thot ra
CH 3 N N Cl H 2O CH 3OH N 2 HCl
KT QU TH NGHIM
Th nghim 3: Phn ng ca anilin vi HNO2
0.05 g 2-naphtol
0,5 mL aniline + 3 mLNaOH
10%
2 mL HCl 10% Quan st
hin tng
Lm lnh bng Phn cn li
nc vo
NaNO2 20%
Sm phm
un nng Quan st
mui
50oC hin tng
diazonium
KT QU TH NGHIM
GII THCH HIN TNG
Do Anlin (C6H5NH2) l amin thm bc 1 nn khi
phn ng vi HNO2 (NaNO2 + HCl) to ra mui
diazonium c mu vng nht.
t = 0 - 50C
KT QU TH NGHIM
GII THCH HIN TNG
Mui diazonium phn hy to thnh N2 si bt

mnh khi un cch thy 50oC.
to =50oC
+ H2O + N2 + HCl
KT QU TH NGHIM
GII THCH HIN TNG
Mui diazonium tc dng vi -naphtol v

NaOH to ra dung dch c mu da cam.
+ NaOH
+
Th nghim 4: Phn ng ca amin bc 2.
Chit
0,5ml N,N- (vo phu cha
diphenylanilin 20ml ete)
2ml HCl 10% NaOH 10%
Lm lnh Ra
(bng nc )
Loi ete
NaNO220%
(a ng nghim vo
cc nc si t
Bn sn phm trong t ht)
(mu vng y
ng nghim)
Nitrozamin
KT QU TH NGHIM
Diphenylanilin l amin thm bc 2 , khi cho tc

dng vi acid nitro (HNO2) s to ra nitrozamin
mu vng di y ng nghim v nc.
+ NaNO2 + HCl
NHN BIT AMIN BNG PHN NG LIBEMAN
Phn
nitrozamin Nc mu
0,5ml phenol
un Pha long
(nh trong 30 giy)
NaOH 10%
ngui Mu xanh
1ml H2SO4
Kho st th
Bn sn phm
nghim l
(mu xanh thm)
amine bc 2
KT QU TH NGHIM
KT QU TH NGHIM
S th nghim phn ng nhn bit amin bc 2
ng nghim Pyrex: ng nghim Pyren:

N,N-diphenylamin, phenol, H2SO4,pha
HCl, lm lnh, NaNO2 long, NaOH
KT QU TH NGHIM
0,5 mL N,N-
dimetylanilin Lm lnh
(bng nc )
2 ml HCl 10%
Lm lnh Kim ha dd
(bng nc ) (bng NaOH 10%
hoc Na2CO3 10%)
NaNO2 20%
(tng git)
Quan st Amin
Kt ta
(dd c mu ) bc 3
xanh
KT QU TH NGHIM
Cho dung dch NaNO2 20% vo ng nghim

ang lm lnh => chuyn sang mu
+ NaNO2 + 2HCl + NaCl + H2O
KT QU TH NGHIM
Sau lm lnh dd bng nc v kim ha

bng dd NaOH long hoc dd Na2CO3 10%
thy xut hin kt ta xanh.
+ Na2CO3 + 2NaCl + CO2 + H2O
(mu xanh)
KT QU TH NGHIM
Th nghim 8: Phn ng vi ninhidrin
1ml dd protit
2-3 git ninhidrin
Lc nh
un si
(vi pht)
Quan st
(mu dung dch)
KT QU TH NGHIM
GII THCH HIN TNG
Di tc dng ca ninhydrin, acid amin oxi ho b gim

bt mt nguyn t C, n tr thnh dng aldehyde ri n
ammoniac v carbonic, acid amin.
Nh vy cc acid amin c khi lng phn t nh hn

v amoniac to thnh s oxi ho ninhydrin to nn
phc hp c mu xanh tm, cng mu tng ng
vi gi tr nit amin ca acid amin.
KT QU TH NGHIM
GII THCH HIN TNG
KT QU TH NGHIM
GII THCH HIN TNG
KT QU TH NGHIM
Th nghim 6: Phn ng oxi ha monosaccarit bng phc
cht ca bc vi ammoniac
iu ch thuc th Tollens
Thuc th Tollens l dung dch bc nitrat trong amoniac
AgNO3 + NH4OH Ag2O + NH4NO3 + H2O
Kt ta en
Ag2O + NH4OH [Ag(NH3)2]OH
Phc bc amiacac
2ml dd AgNO3 1%
NH3 5%
(tng git) Ha tan hon ton kt ta
Ag2O
Thuc th Tolen
(Phc bc amiacat
[Ag(NH3)2]OH)
1ml dd frutozo 1ml dd glucozo
1% 1%
un nng un nng
(khng lc ng nghim) (khng lc ng nghim)
Quan st hin
tng
KT QU TH NGHIM
GII THCH HIN TNG
Thuc th Tollens dng oxi ha -CHO ca

glucozo dng mch h thnh axit -COOH v gii
phng ra Ag kim loi.
Phng trnh ha hc ca glucoz vi phc amiacac

CH2OH(CHOH)4CHO + 2[Ag(NH3)2]OH CH2OH[CHOH]4COONH4
+ 2Ag + 3NH3 + H2O
GII THCH HIN TNG
Trong mi trng OH- Fuctozo b chuyn thnh

glucozo nn cng c kh nng tham gia phn ng
trng gng.
TN 7: Phn ng oxi ha monosaccarit bng ng (II) hydroxit
2ml glucozo 1%, 2ml frutozo1%,

1ml NaOH 10% 1ml NaOH 10%
CuSO4 5%
CuSO4 5%
(Tng git)
(Tng git)
Lc u Lc u
(ti bt u vn c) (ti bt u vn c)
un nng (n bt u un nng (n bt u
si -phn trn) si -phn trn)
Quan st (kt ta Quan st (kt ta

mu gch) mu gch)
KT QU TH NGHIM
Glucoz phn ng Cu(OH)2
+ 2CuSO4 + NaOH
+ Cu2O + H2O
gch
KT QU TH NGHIM
Fuctozo phn ng Cu(OH)2
Trong mi trng OH- fuctoz chuyn ha thnh glucoz
Fuctozo OH- glucozo
CH2OH(CH2OH)4CHO + 2CuSO + NaOH HOCH (CHOH) COONa

4 2 4
+ Cu2O + H2O
gch
KT QU TH NGHIM
Lu
Khng c d v khng
c thiu CuSO4 v:
D: Cu(OH)2 CuO + H2O

mu en
Thiu: glucozo b ha nha khi un nng
Kh quan st mu sc ca kt ta
TN 8: Phn ng oxi ha monosaccarict bng
thuc th Felling
500ml H2O 1ml
glucozo Quan st
(mu kt
34,6g ta)
Feling A
CuSO4.5H2O
Lc u
un nng
Thuc th un nng (bt u si)
(bt u si)
Feling
173g mui Quan st Lc u

natri kali (mu kt
Feling B
tactrat, ta)
70g NaOH
500ml H2O 1ml
frutozo
KT QU TH NGHIM
GII THCH HIN TNG
Glucozo tc dng vi thuc th felling th ta thy xut

hin kt ta gch ca Cu2O do glucozo b felling kh
nhm chc CHO thnh nhm chc -COOH
Phng trnh phn ng
Cu(OH)2 + NaOH
CH2OH(CH2OH)4CHO +
NaOOC(CHOH)2COOK
Cu2O + HOCH2(CHOH)4COONa
KT QU TH NGHIM
KT QU TH NGHIM
GII THCH HIN TNG
Trong mi trng OH- Fuctozo b chuyn thnh

glucozo nn cng b kh bi thuc th Felling
tng t glucozo.
TN 9: Phn ng oxi ha monosacarit bng nc brom
1 mL dd 1 mL dd
Brom Brom
un trong ni nc si 15 pht
3 mL
dung dch
Phenol 1% Quan st Quan st hin
Dd FeCl3 1% hin tng tng
Phc phenol st
ngui dung dch
(III) Clorua
(Phc mu xanh tm)

Quan st Quan st hin
hin tng tng
KT QU TH NGHIM
GII THCH HIN TNG
Glucozo lm mt mu nc brom( glucozo: th hin tnh

kh) v to phc mu vng xanh vi ion Fe3+
Phng trnh glucoz phn ng vi dung dch Brom
CH2OH-(CHOH)4-CH=O + Br2 + H2OCH2OH-(CHOH)4-COOH + HBr
Phng trnh gluconic acid phn ng vi phc phenol- FeCl3
CH2OH-(CHOH)4-COOH + [Fe(OAr)6]3- + H+
C6H5OH +
[CH2OH-(CHOH)4-COO]3Fe
(Vng hi xanh)
KT QU TH NGHIM
Frutozo khng lm
mt mu brom v
khng phn ng vi
phc phenol- FeCl3
gii thch ?
TN 10: Phn ng thy phn saccararozo, tinh
bt trong mi trng axit
Phn ng thy
1,5 mL dd
sacarozo 1% phn saccararozo
5 mL dd H2SO4 10%
un si 2 -3 pht Quan st hin

tng v gii
thch
Lm lnh
un phn trn
ca ng nghim
NaHCO3 tinh th
Khuy hn hp 2 mL dd Felling
(ht CO2)
KT QU TH NGHIM
GII THCH HIN TNG
Trong mi trng acid, saccararozo thy phn to thnh

glucozo v fructozo:
H+
C12H22O11 + H2o C6H12O6 + C6H12O6
tC
Glucozo Fructozo
Glucozo phn ng vi thuc th Feling to kt ta g gch
CuO2(glucozo: th hin tnh kh):
O-CH-COONa H+
CH2OH-(CHOH)4-CH=O + Cu CuO2
+
O-CH-COOK toC
HO-CH-COONa
CH2OH-(CHOH)4-COOH +
HO-CH-COOK
KT QU TH NGHIM
1g tinh bt
9-10ml
nc si
Khuy u
Nc
nh mc
(V=49ml)
Dd h tinh bt
2%
KT QU TH NGHIM
Phn ng thy phn
h tinh bt
4 mL dd h tinh
bt 2%
1 mL H2SO4 10%
Lc u 1 mL dd I2
trong KI Trung ha bng
NaOH 10%
2 git sp
un trong bp Dd felling
cch thy 20 pht
Dung dch Quan st
mu vng hin tng
Sn phm sng
(keo => lng)
KT QU TH NGHIM
GII THCH HIN TNG
H tinh bt thy phn trong mi trng acid to tri

qua cc giai on sau:
(C6H10O5)n + nH2O H+ (C6H10O5)m C12H22O11 C6H12O6
(Dextrin) (Mantozo) (glucozo)
Glucozo phn ng vi thuc th Feling to kt ta g

gch CuO2 (glucozo: th hin tnh kh):
O-CH-COONa H+
CH2OH-(CHOH)4-CH=O + Cu CuO2 +
O-CH-COOK toC
HO-CH-COONa
CH2OH-(CHOH)4-COOH +
HO-CH-COOK
Th nghim 11 : Phn ng ca tinh bt vi iot
CHC CC BN HC TT !
Bi thuyt
trnh ca
nhm n
y l kt
thc.
Cm n C
v cc bn
BYE BYE
ch
lng nghe .
Thn cho
THANK YOU!
C cng cc
bn! 46

Báo Cáo TH C Hành Hóa Hưu Cơ Nhóm 1

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Báo Cáo TH C Hành Hóa Hưu Cơ Nhóm 1

Uploaded by

Copyright:

Available Formats

KHOA: CNG NGH HA HC

Gii thch hin tng

Metylamin c tnh bazo nn khi cho

2ml HCl 10%

Amin bc 1 (metylamin-CH3NH2) tc dng vi

HNO2(NaNO2+HCl) to ra mui diazonium.

Mui diazonium trong mi trng c to = 0-5oC s

Mui diazonium phn hy to thnh N2 si bt

Mui diazonium tc dng vi -naphtol v

Diphenylanilin l amin thm bc 2 , khi cho tc

ng nghim Pyrex: ng nghim Pyren:

Cho dung dch NaNO2 20% vo ng nghim

+ NaNO2 + 2HCl + NaCl + H2O

Sau lm lnh dd bng nc v kim ha

+ Na2CO3 + 2NaCl + CO2 + H2O

2-3 git ninhidrin

Di tc dng ca ninhydrin, acid amin oxi ho b gim

Nh vy cc acid amin c khi lng phn t nh hn

Thuc th Tollens dng oxi ha -CHO ca

Phng trnh ha hc ca glucoz vi phc amiacac

Trong mi trng OH- Fuctozo b chuyn thnh

2ml glucozo 1%, 2ml frutozo1%,

Quan st (kt ta Quan st (kt ta

CH2OH(CH2OH)4CHO + 2CuSO + NaOH HOCH (CHOH) COONa

D: Cu(OH)2 CuO + H2O

Thiu: glucozo b ha nha khi un nng

173g mui Quan st Lc u

Glucozo tc dng vi thuc th felling th ta thy xut

Trong mi trng OH- Fuctozo b chuyn thnh

(Phc mu xanh tm)

Glucozo lm mt mu nc brom( glucozo: th hin tnh

Phng trnh gluconic acid phn ng vi phc phenol- FeCl3

un si 2 -3 pht Quan st hin

Trong mi trng acid, saccararozo thy phn to thnh

H tinh bt thy phn trong mi trng acid to tri

(Dextrin) (Mantozo) (glucozo)

Glucozo phn ng vi thuc th Feling to kt ta g

You might also like