Scribd Logo
Upload

Welcome to Scribd!

  • Mikro Industri Insulin

    Uploaded by

    Rara
    23 views9 pages
    The document summarizes the process of producing insulin from E. coli, including upstream processing, fermentation, and downstream processing. Upstream processing involves preparing media and equipment like LB broth, inoculating transformed bacteria, and setting up bioreactors. Fermentation occurs first in small scale shake flasks containing antibiotics, then in large scale fermentors under optimal temperature and pH conditions while being monitored. Downstream processing isolates crude products, purifies the crude product, obtains insulin chains, and synthesizes active insulin, which is then purified through PR-HPLC to obtain highly purified insulin.

    Original Description:

    Presentasi mata kuliah Mikrobiologi Industri Departemen TPHP, FTP, UGM

    Copyright

    © © All Rights Reserved

    Available Formats

    PPTX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    The document summarizes the process of producing insulin from E. coli, including upstream processing, fermentation, and downstream processing. Upstream processing involves preparing media and equipment like LB broth, inoculating transformed bacteria, and setting up bioreactors. Fermentation occurs first in small scale shake flasks containing antibiotics, then in large scale fermentors under optimal temperature and pH conditions while being monitored. Downstream processing isolates crude products, purifies the crude product, obtains insulin chains, and synthesizes active insulin, which is then purified through PR-HPLC to obtain highly purified insulin.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pptx, pdf, or txt
    23 views9 pages

    Mikro Industri Insulin

    Uploaded by

    Rara
    The document summarizes the process of producing insulin from E. coli, including upstream processing, fermentation, and downstream processing. Upstream processing involves preparing media and equipment like LB broth, inoculating transformed bacteria, and setting up bioreactors. Fermentation occurs first in small scale shake flasks containing antibiotics, then in large scale fermentors under optimal temperature and pH conditions while being monitored. Downstream processing isolates crude products, purifies the crude product, obtains insulin chains, and synthesizes active insulin, which is then purified through PR-HPLC to obtain highly purified insulin.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pptx, pdf, or txt
    of 9

    INSULIN PRODUCTION

    FROM Escherichia coli


    ANDI SUARES
    ANANG WAHIF FATKHURROCHMAN
    MUHAMMAD GAUSSIAN
    AUFA NAUFALIA NABILA
    ALFRISTA RURI NARESWARI
    MUTIARA NUR ISNAINI
    UYUN NURUL AINI
    INTRODUCTION
    UP-STREAM PROCESSING
    Media & equipment preparation:
    The LB broth is prepared It is
    antoclaved and ampicillin and
    lactose are added (after the
    sterilization to prevent denaturation or
    destruction). Inoculation is done by
    adding the transformed bacteria into the
    media.
    Preparation of the bioreactor Parts of
    the bioreactors are fixed and checked
    such as the calibration of the pH
    electrode, pO2 probe, exhaust
    condensers and air inlet. The
    FERMENTATION

    Small Large
    scaling scaling
    (enrichmen (fermentor)
    t liquid
    culture in
    shake
    flask)
    Contains Optimal conditions:
    ampicillin and - Temperature
    lactose as - pH
    antibiotic.
    Monitoring &
    MANUFACTURE CONTROL

    Cleanliness

    Sterility

    Purity
    DOWNSTREAM PROCESSING
    Isolation of crude products

    Purification of crude product

    Obtaining of insulin chains

    Synthesis of active insulin

    PR-HPLC to obtain highly purified insulin


    TERIMA KASIH

    You might also like