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LC (Lethal Concentration) : Uji Toksisitas Kuantitatif
LC (Lethal Concentration) : Uji Toksisitas Kuantitatif
LC (Lethal Concentration) : Uji Toksisitas Kuantitatif
100%
Response
0%
1. Organisme Uji
Artemia, Dhapnia : Acute Test
Ikan Mas, Udang : Acute, Sub-Lethal
V1.N1 = V2.N2
x.1000 = 100.100
PA PS H
Original Concentration
PA PS H
V1.N1 = V2.N2
100 g/mL
x.100 = 10.10
Diambil 1 mL
+ 9 mL medium
Larutan Stock
0,1 gL
10 -3
1. Static Test
Expose to The Test Medium 2. Renewable Test
3. Resirculation Test
4. Flow-Trough Test
Observe Mortality
for Determine LC50
3
Determine LC50 Using Probit Analysis
1
Metode Hubert (1979)
m= 5a
b . (1)
b = XY 1/ n ( X Y)
X2 1/ n ( X)2 . (2)
a = 1/ n (Y b X) ..(3)
Normal distribution of resistance/sensitivity
100
Mean response
Frequency
Protected
5% allowable
impact
0
Preparing Data Tabulation Lihat di Tabel
Probit % Mortalitas
Format Tabulasi Data Analisis Probit LC50
Jenis Hewan Uji :
Jenis Bahan Toksik :
d n r p X Y XY
(Konsentrasi (jumlah (Mortalitas (%Mortalitas) (Log (Nilai Probit %
Uji) hewan uji) hewan uji) Konsentrasi) Mortalitas)
i 30
ii 30
iii 30
Jumlah ( ) X Y XY
Log Probit
1.009 6.18
6.18
1.009
Determine a,b and m value to determine LC50 value
b = .. Persamaan (2)
a = . Persamaan (3)
m = . Persamaan (1)
Dose-Response
1. Use inverse prediction from the graph to estimate the Log 10 of the LC84, LC16,
and LC50 then un-log the values and express as mg/L.
Log10 Conc. Conc. (mg/L)
eg: LC84 = .93 8.51
LC16 = .43 2.69
LC50 = .68 4.79
N = 49 + 46 + 48 = 143
5. Calculate Upper and Lower 95% Confidence Limits (multiply or divide 'unlogged'
conc/dose values by f):
Case Sample :
Data Mortalitas Larva Udang Windu Setelah Pemaparan 96 jam pada Logam Cu
0 (Kontrol) 30 0
0.251 30 0
0.63 30 3
1.58 30 13
3.97 30 19
9.96 30 30
LC_Cu_UdangWindu
Proportion
Observed Responding Predicted
Number Number Proportion Adjusted for Proportion
Conc. Exposed Resp. Responding Controls Responding
Mu = 0.330260
Sigma = 0.388480
Photo by R. Grippo