OPTIMIZATION OF SPRAY DRYING PROCESS TO

IMPROVE VIABILITY OF PROBIOTIC WITH

BACILLUS SUBTILIS

PROPONENTS: CRISTAL MAE BALAGA

SHENAR MOERA JOY RESURRECCION
BETHEL GRACE TALHA
ADVISER: MA. SHEILA K. RAMOS, PHD
PROBIOTICS
BACILLUS SUBTILIS

Bacillus subtilis is a rod-

shaped, Gram-positive
bacterium that is normally
found in soil. As a member of
t he s por e- for mi ng genus
Bacillus, it can survive in
extreme environments such as
with high temperatures.
PROBLEM

Spray drying is widely studied and used in large processes because it is energy
efficient and cheap. However, research shows spray drying decreases probiotic
yield because it cant survive the high-temperature environment. Because of
low survival rate, studies on probiotic products have shown that some do not
contain probiotic at all. The current study aims to optimize the spray drying
process by varying inlet temperature, and coating ratio in order to improve the
viability of probiotic from Bacillus subtilis.
OBJECTIVES

The general objective of the study is to optimize the spray drying process
for the production of probiotic powder from Bacillus subtilis, specifically,
it aims

To determine the optimum inlet temperature

To determine optimum maltodextrin-alginate-starch ratio
To determine viability of probiotic after spray drying process
METHODOLOGY

Growth of
Homogenization
Bacillus subtilis

Measurement of
Percent Viability Spray Drying
RESPONSE SURFACE METHODOLOGY
Runs Inlet Temperature (degrees Celsius) Maltodextrin-Alginate-Starch Ratio
1 100 2:1:1
2 120 2:1:1
3 110 1:1:1
4 110 1:1:2
5 110 2:1:1
6 120 2:1:1
7 100 1:1:2
8 100 1:2:1
9 100 1:1:1
10 120 1:2:1
11 120 1:1:2
12 110 1:2:1
13 100 1:1:1
14 100 1:1:2
15 100 1:2:1
16 120 1:1:2
17 100 2:1:1
18 120 1:1:1
19 120 1:1:1
20 120 1:2:1