Detection and Segmentation of

Cell Nuclei
In Histopathology Images
 Objective
To understand the Image Segmentation Algorithms

To develop efficient algorithms for detecting and

segmenting cell nuclei in 2-D histopathology images.
 Introduction
Detecting Neuclii in a histopathology images is normally
the first step towards counting cells, quantifying
molecular markers in healthy and pathologic specimen
Algorithm used for segmentation depends on imaging
modalities, staining procedures, experimental conditions
etc.
Many computational methods have been developed
and applied to cell nuclei segmentation in 2D images.
High accuracy in detecting cell neuclii is desired
 Segmentation.. Methods
Thresholding
Morphological
Watershed
Region Growing
Active Contour Model
Clustering
Graph Cut
Proposed Method
 Steps Involved

1. Automatic Image Binarization

2. Seed Detection and Initial Segmentation
3. Refinement of Initial Nuclear Segmentation using
α-Expansions and Graph Coloring
4. Computer assisted editing of segmentation results.
Expected Result
 References
1. Al-Kofahi Y, Lassoued W, Lee W, Roysam B. Improved automatic detection
and segmentation of cell nuclei in histopathology images. IEEE Trans
Biomed Eng 2010;57:841–852.
2. Cheng Chen, Wei Wang, John A. Ozolek, Gustavo K. Rohde. A Flexible and
Robust Approach for Segmenting Cell Nuclei from 2D Microscopy Images
Using Supervised Learning and Template Matching. Cytometry A. 2013 Apr
8.
3. Humayun Irshad, Student Member, IEEE, Antoine Veillard, Ludovic Roux, and
Daniel Racoceanu, Member, IEEE. Methods for Nuclei Detection,
Segmentation, and Classification in Digital Histopathology: A Review -
Current Status and Future Potential. IEEE Reviews in Biomedical Engineering
2014, Vol 7: 97-114