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Stoichiometry of Cell Growth and Product Formation
Stoichiometry of Cell Growth and Product Formation
of Cell
Growth and
Product
Formation
Law of Conservation
of Mass
Available electrons
- number of electrons available for
transfer to oxygen on combustion of a
substance to CO2, H2O, and nitrogen-
containing compounds.
For substrate CwHxOyNz:
Number of available electrons = 4𝑤 + 𝑥 − 2𝑦 − 3𝑧
III.Electron Balances
Degree of reduction, γ,
- the number of equivalents of available
electrons in that quantity of material
containing 1g atom carbon.
4𝑤 + 𝑥 − 2𝑦 − 3𝑧
𝛾𝑆 =
𝑤
III.Electron Balances
𝑤𝛾𝑆 − 4𝑎 = 𝑐𝛾𝐵
IV.Biomass Yield
g cells produced
𝛾𝑋𝑆 =
g substrate consumed
𝑐(MW cells)
𝛾𝑋𝑆 =
MW substrate