EXPERIMENT NO.

5
 Hydrolysis

starch sucrose
weigh weigh
0.5 g 0.5 g
dissolve in dissolve in

6 ml distilled 6 ml distilled
water water
- mix well - mix well
- divide equal - divide equal

tt1 tt2 tt3 tt1 tt2 tt3

add add add add add add
2ml 3M HCl, NaOH 2 ml water, NaOH2 ml water 3M HCl, NaOH water, NaOH 2 ml water
leave
room temp.
Δ (30 minutes) room temp. Δ (30 minutes)
water bath, remove and cool water bath, remove and cool
 Test

Benedict Iodine Seliwanoff

+I2 in KI

+ Seliwanoff
tt1. Starch
+ drops Benedict's

tt1. Starch tt1. Sucrose

solution
tt2. Starch Δ, 2 mins. tt2. Starch
solution

tt3. Starch tt3. Starch tt2. Sucrose

tt1. Sucrose tt3. Sucrose

tt2. Sucrose
Δ, 3 mins.
tt3. Sucrose

Δ, 3 mins.

Observe and compare results

Sample Tube Treatment Test Observation Inference
Blue colored solution Starch was
Benedict’s Test turned to dark hydrolyze.
+ HCl, Δ boiling orange-red colored
A water bath, + solution
NaOH Cloudy white colored Starch was
S solution turned to hydrolyze.
Iodine Test light gray colored
T solution
Blue colored solution Starch was not
A Benedict’s Test remained the same hydrolyzed
+ HCl, Δ boiling Cloudy white colored Starch was not
R B water bath, solution turned to hydrolyze.
+H2O Iodine Test dark blue-violet
C colored solution
Blue colored solution Starch was not
H Benedict’s Test remained the same hydrolyze.
+H2O, Room Cloudy white colored Starch was not
C Temperature, solution turned to hydrolyze.
+H2O Iodine Test light violet colored
solution
Sample Tube Treatment Test Observation Inference
Blue colored solution Sucrose was
+ HCl, Δ boiling Benedict’s Test turned to orange hydrolyze.
water bath, + colored solution
A NaOH
Golden yellow colored Sucrose was
S Seliwanoff ’s Test solution turned to hydrolyze.
maroon colored
U solution
Blue colored solution Sucrose was not
C + HCl, Δ boiling Benedict’s Test turned to orange-brown hydrolyze.
water bath, +H2O colored solution
R B
Faint yellow colored Sucrose was not
O Seliwanoff ’s Test solution turned to clear hydrolyze.
red colored solution
S
Blue colored solution Sucrose was not
E +H2O, Room Benedict’s Test turned to orange-brown hydrolyze.
Temperature, colored solution
C +H2O
Faint yellow colored Sucrose was not
Seliwanoff ’s Test solution turned to clear hydrolyze.
red colored solution
1. In which tube (A, B, C) have you observed hydrolysis of starch and sucrose? Prove
your answer.
Starch: In Tube A the hydrolysis of starch was observed. Heating of starch in the
presence of concentrated HCl causes its hydrolysis into
glucose. Glucose reacts with Benedict’s reagent and
orange-red color was observed in a positive result. Iodine test is a
test for the presence of starch in which sample turns blue-black in
color. However, since starch was broken down or
hydrolysed , blue-black was not observed. Therefore iodine test
indicate the completion of hydrolysis in starch when
blue-black color is not observed.