Professional Documents
Culture Documents
2018 - Lecture 3-Canvas
2018 - Lecture 3-Canvas
2018 - Lecture 3-Canvas
A) HSP-70
B) Ubiquitin Activating Enzyme
C) E2
D) E3
E) Proteasome core
Experimental Question:
You have a drug that blocks the activity of one of the
enzymes involved in ubiquitylation of proteins. You treat cells
with this drug and you determine the effect of treatment on
the abundance of different proteins by Western blot analysis.
Cdc2 is a control of a protein that is not ubiquitylated. From
the literature you know that the other 4 proteins are
ubiquitylated by different E2+E3 ligase proteins.
YFP
0 6 12 hours
Based on this Western blot you can conclude that:
A = proteins larger than YFP are synthesized
B = drug treatment does not affect YFP in any way
C = drug treatment causes YFP to be destroyed in the proteasome
D = drug treatment causes YFP to become ubiquitylated
E = drug treatment caused the accumulation of ubiquitylated-YFP
Experimental Question:
How can you show that the bands larger than YFP represent
polyubiquitylated forms of YFP?
YFP
0 6 12 hours
B. Different types of such aggregates can form from the same protein.
E. Some healthy cells form these aggregates to store their secretory proteins.
Lecture 3 DNA, chromosome and Genome-
• Gene:
• Double strand (how to read)
• DNA duplication
• How to improve DNA is heritable?
• Chromatin:
• Histone
• Histone modification
• Human genome
discovered in 1953 by Watson and Crick at the University of Cambridge
, sing experimental data collected by Rosalind Franklin and
Maurice Wilkins. The structure of DNA is non-static,[9]
all species comprises two helical chains each coiled round the same axi
s, and each with a pitch of 34
Adenine Thymine
Guanine Cytosine
purine pyrimidine
• Indicate which numbered feature (1 to 5) in the schematic
drawing below of the DNA double helix corresponds to each
of the following. Your answer would be a five-digit number
composed of digits 1 to 5 only, e.g. 52431.
( ) Hydrogen-bonding
( ) Covalent linkage
( ) Phosphate group
( ) Nitrogen-containing base
( ) Deoxyribose sugar
• Indicate which numbered feature (1 to 5) in the schematic
drawing below of the DNA double helix corresponds to each
of the following. Your answer would be a five-digit number
composed of digits 1 to 5 only, e.g. 52431.
(4) Hydrogen-bonding
(3) Covalent linkage
(2) Phosphate group
(1) Nitrogen-containing base
(5) Deoxyribose sugar
THE STRUCTURE AND FUNCTION
OF DNA
In living organisms DNA does not usually exist as a single molecule, but instead as
a pair of molecules that are held tightly together
The Structure of DNA Provides a Mechanism
for Heredity
1. semi conservative
2. 5’-3’ synthesis
3. 3’-5’ proofreading
DNA polymerases have
proofreading abilities.
Chromosomal DNA and Its Packaging in the
Chromatin Fiber
B. Different types of such aggregates can form from the same protein.
E. Some healthy cells form these aggregates to store their secretory proteins.