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    Bacteriology Inventory

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    Princess Gwen Zman
    This document discusses the Gram staining technique used to classify bacteria as either Gram positive or Gram negative. Gram positive bacteria appear blue under the microscope due to their thick peptidoglycan cell wall trapping the primary stain. Gram staining is used to assess sputum specimens for quality before performing bacterial cultures, with specimens containing over 25 epithelial cells per field being rejected as "spit" rather than sputum.

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    Bacteriology Inventory

    Uploaded by

    Princess Gwen Zman
    8 views11 pages
    This document discusses the Gram staining technique used to classify bacteria as either Gram positive or Gram negative. Gram positive bacteria appear blue under the microscope due to their thick peptidoglycan cell wall trapping the primary stain. Gram staining is used to assess sputum specimens for quality before performing bacterial cultures, with specimens containing over 25 epithelial cells per field being rejected as "spit" rather than sputum.

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    ABOUT ABOUT BACTERIOLOGY

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    This document discusses the Gram staining technique used to classify bacteria as either Gram positive or Gram negative. Gram positive bacteria appear blue under the microscope due to their thick peptidoglycan cell wall trapping the primary stain. Gram staining is used to assess sputum specimens for quality before performing bacterial cultures, with specimens containing over 25 epithelial cells per field being rejected as "spit" rather than sputum.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pptx, pdf, or txt
    8 views11 pages

    Bacteriology Inventory

    Uploaded by

    Princess Gwen Zman
    This document discusses the Gram staining technique used to classify bacteria as either Gram positive or Gram negative. Gram positive bacteria appear blue under the microscope due to their thick peptidoglycan cell wall trapping the primary stain. Gram staining is used to assess sputum specimens for quality before performing bacterial cultures, with specimens containing over 25 epithelial cells per field being rejected as "spit" rather than sputum.

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    Bacteriology

    COUNTER Stain Safranin


    Gram Positive
    • Primary Stain
    • Mordant
    • Decolorization
    • Counter Stain
    Gram Negative
    • Peptidoglycan in positive cell wall traps the
    crystal violet, hence blue color.
    Gram positive cocci in pairs and chains
    streptococccus
    Gram Negative bacillus resembling an
    enteric
    Gram Positive rod most likely a bacillus
    species
    Gram positive cocci in clusters
    staphylococcus
    Gram Negative rod-fusiform shaped-
    fusobacterium species
    Gram Stain
    • Gram Stan is used to assess quality of sputum
    specimen for culture.
    • Expectorated sputum specimen is rejected for
    bacterial culture if judged to be “spit”
    -perform gram stain of sputum specimen
    -if >=25 epithelial cells/ field
    -sputum is judged to be spit/ not a sputum
    specimen
    -bacterial culture is not performed/ it is
    rejected
    -

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