Professional Documents
Culture Documents
DNA Recombination: - Roles - Types - Homologous Recombination in E.coli - Transposable Elements
DNA Recombination: - Roles - Types - Homologous Recombination in E.coli - Transposable Elements
• Roles
• Types
• Homologous recombination in E.coli
• Transposable elements
Biological Roles for Recombination
Fig. 22.1
Holliday Model
R. Holliday (1964)
- Holliday Junctions
form during
recombination
Fig. 22.3
The recBCD
Pathway of
Homologous
Recombination
• RecBCD has:
1. Endonuclease subunits (recBCD) that cut
one DNA strand close to Chi sequence.
2. DNA helicase activity (recBC subunit) and
3. DNA-dependent ATPase activity
– unwinds DNA to generate SS regions
Fig. 22.6
RecA Function Dissected
Fig. 22.31a
Transposable Elements
(Transposons)
• DNA elements capable of moving ("transposing")
around the genome
• Discovered by Barbara McClintock, largely from
cytogenetic studies in maize, but since found
in most organisms
• She was studying "variegation" or sectoring in
leaves and seeds
• She liked to call them "controlling elements“
because they effected gene expression in
myriad ways
Mutant Kernel Phenotypes
• Pigmentation mutants
– affect anthocyanin pathway
– elements jump in/out of transcription
factor genes (C or R)
– sectoring phenotype - somatic mutations
– whole kernel effected - germ line mutation
Fig. 23.19
Other Characteristics of McClintock's
Elements
Duplication
remains when
element excises,
thus the
Footprint.
Mutator (A Retrotransposon)