Complete Blood Count

Ian Gabrielle M. Cuyno, RMT

Complete Blood Count
Hemoglobin, Hematocrit
RBC count with Indices
WBC count with Differential
Platelet count (or Estimate)
Manual CBC Procedure:
 Microhematocrit
 Hemoglobinometry
 Manual cell counts (RBC, WBC, Platelet)
 Manual Differential Count
 Platelet estimate
Hct
Manual Cell counts
 For RBC, WBC, Platelet
Blood Smear
Diff Count
Semi-Automated CBC
 Automated Hgb, Hct, Cell counts,
Indices, etc.
 Peripheral Blood Smear (for evaluation)
 WBC Differential Count
 Platelet count
RBC Count
Hematocrit
 The volume/ or ratio of packed red cells that occupies
a given volume of whole blood
 Expressed as percentage (45%) or in Liters per Liter
(0.45 L/L)
 May also be used as indirect assessment of hydration
status
Hemoglobin
 The weight of hemoglobin per volume of whole blood
 Measure of the O2 carrying capacity of blood
 Determined in grams per deciliter (13.5 g/dL) or grams
per liter (135 g/L)
 “Rule of 3”

DISCLAIMER:
 Do not use this formula to obtain either value
 Only for quick validation/double checking
RBC Indices
 Calculated values
 Useful in morphologic classification
of anemias
Mean Cell Volume

Mean Cell Hemoglobin

Mean Cell Hemoglobin

Concentration
Mean Cell Volume

MCV < 80 fL MCV 80-100 fL MCV >100 fL

Microcytic Normocytic Macrocytic
Mean Cell Hemoglobin Concentration

MCHC <32 g/dL MCHC 32-36 g/dL MCHC >36 g/dL

Hypochromic Normochromic Hyperchromic*
MCV MCHC
Morphology Example
(fL) (g/dL)
Iron Deficiency Anemia,
<80 <32 Microcytic, Hypochromic
Thalassemia
Aplastic Anemia, Hemolytic
80-100 32-36 Normocytic, Normochromic
Anemia
Megaloblastic Anemia, Liver
>100 32-36 Macrocytic, Normochromic
Disease
Red Cell Distribution Width (RDW)
 Index of Anisocytosis (Variation in cell size)
 Machine derived; based on SD or CV of cell sizes
Red Cell Distribution Width

Normal RDW Anisocytosis:

11.5 – 14.5% Variation in cell size
RDW: >14.5%