Practical: no 1

Study of Cytochemical Detection of

DNA in Protozoa or Avian Blood Cell.
 Practical no 1
• Practical:
• . STUDY OF CYTOCHEMICAL DETECTION OF DNA IN
PROTOZOA OR AVIAN BLOOD CELL.
• MATERIAL:
• 1. Protozoa Culture 6. Glass slides 11. Avian blood
• 2. Aceto-orcein stain 7. Cover slips 12. Formo-saline
• 3. Acetone 8. Distilled Water 13. Syringe
• 4. Glycerine 9. Light Microscope 14. Heparin
• 5. Methyl green pyronin 10.Acetic acid 15. Xylene
•
• Cytochemical Study:
• The study of chemical components of the cell
without extracting DNA from the cells.
• Culture Preparation:
• Collect leaf litters under plants and put them
in a dish containing stagnant water. Place the
dish under shady place at room temperature.
Wait for 2-3 days for the preparation of
protozoa culture.
• TECHNIQUE:
• For Protozoa Culture
•
• 1. Wash hands and put on gloves.
• 2. Put a protozoa culture drop on the slide.
• 3. Add a drop of acetic acid (for 2 minutes) to fix the protozoa.
• 4. Wash the slide with distilled water.
• 5. Put a drop of methyl green pyronin stain for 15 minutes.
• 6. Again wash with distilled water and then dehydrate it with
acetone.
• 7. Finally wash it with xylene.
• 8. Mount the slide with glycerine.
• 9. Observe the green blue color of DNA under the microscope.
•
• For Avian Blood Cells

• 1. Take any bird blood in a syringe containing anticoagulant (heparin or

sodium citrate).
• 2. Put a drop of blood on slide and make a thin smear.
• 3. Dry the blood smear in air.
• 4. Add few drops of formo-saline (for 4 minutes) to fix the blood cells.
• 5. Put few drops of methyl green pyronin stain for 15 minutes.
• 6. First wash with distilled water and then dehydrate it with acetone.
• 7. Finally wash it with xylene.
• 8. Mount the slide with glycerine.
9. Observe the green color of DNA under the microscope.
•