Scribd Logo
Upload

Welcome to Scribd!

  • 13 views

    Improving The Stability of Glutamate Fermentation

    Uploaded by

    Radhit
    This study investigated using sorbitol or glycerol addition to improve succinate production by Corynebacterium glutamicum ATCC1302 through ATP-based regulation of glucose metabolism. Two methods of addition were tested: 1) co-feeding sorbitol or glycerol with glucose at a 1:5 ratio, and 2) adding 5-15 g/L sorbitol or glycerol to the initial media before inoculation with glucose feeding below 20 g/L. Both methods improved succinate production compared to normal batch fermentation, with method 1 resulting in higher succinate yields. The results suggest sorbitol and glycerol addition enhanced succinate production by regulating glucose metabolism through ATP supply

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content

    You might also like

    Improving The Stability of Glutamate Fermentation

    Uploaded by

    Radhit
    13 views18 pages
    This study investigated using sorbitol or glycerol addition to improve succinate production by Corynebacterium glutamicum ATCC1302 through ATP-based regulation of glucose metabolism. Two methods of addition were tested: 1) co-feeding sorbitol or glycerol with glucose at a 1:5 ratio, and 2) adding 5-15 g/L sorbitol or glycerol to the initial media before inoculation with glucose feeding below 20 g/L. Both methods improved succinate production compared to normal batch fermentation, with method 1 resulting in higher succinate yields. The results suggest sorbitol and glycerol addition enhanced succinate production by regulating glucose metabolism through ATP supply

    Original Description:

    Stability of Glutamate Fermentation

    Original Title

    Improving the Stability of Glutamate Fermentation

    Copyright

    © © All Rights Reserved

    Available Formats

    PPTX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    This study investigated using sorbitol or glycerol addition to improve succinate production by Corynebacterium glutamicum ATCC1302 through ATP-based regulation of glucose metabolism. Two methods of addition were tested: 1) co-feeding sorbitol or glycerol with glucose at a 1:5 ratio, and 2) adding 5-15 g/L sorbitol or glycerol to the initial media before inoculation with glucose feeding below 20 g/L. Both methods improved succinate production compared to normal batch fermentation, with method 1 resulting in higher succinate yields. The results suggest sorbitol and glycerol addition enhanced succinate production by regulating glucose metabolism through ATP supply

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pptx, pdf, or txt
    13 views18 pages

    Improving The Stability of Glutamate Fermentation

    Uploaded by

    Radhit
    This study investigated using sorbitol or glycerol addition to improve succinate production by Corynebacterium glutamicum ATCC1302 through ATP-based regulation of glucose metabolism. Two methods of addition were tested: 1) co-feeding sorbitol or glycerol with glucose at a 1:5 ratio, and 2) adding 5-15 g/L sorbitol or glycerol to the initial media before inoculation with glucose feeding below 20 g/L. Both methods improved succinate production compared to normal batch fermentation, with method 1 resulting in higher succinate yields. The results suggest sorbitol and glycerol addition enhanced succinate production by regulating glucose metabolism through ATP supply

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pptx, pdf, or txt
    of 18

    ATP-Based Ratio Regulation of Glucose and

    Xylose Improved Succinate Production


    Fengyu Zhang, Jiaojiao Li, Huaiwei Liu, Quanfeng Liang,
    Qingsheng Qi
    Department of State Key Laboratory of Microbial Technology, Shandong University, Jinan, P. R.
    China
    PLOS ONE | DOI:10.1371/journal.pone.0157775 June 17, 2016
    Permasalahan
    Landasan Teori
    Hipotesis
    Metode
    Method #1: 50% (w/v) sorbitol atau glycerol
    Main ditambahkan bersamaan dengan feeding
    Seed
    fermentation glukosa. feeding ratio 1:5 (w/w) sorbitol atau
    glycerol versus glukosa
    • C. Glutamicum ATCC1302 • C. Glutamicum ATCC1302
    • 32oC • 1,33 vvm
    • pH 7.0-7.2 • 34-36 jam
    Method #2: 5 to 15 g /L sorbitol atau gliserol
    • 200 rpm • 32oC ditambahkan ke dalam media awal sebelum
    • 8-10 jam • pH 7.0-7.2 inokulasi. Feeding dilakukan ketika konsentrasi
    Seed fermentation contained glukosa lebih rendah dari 20 g / L.
    fermentation contained (in g/L)
    (in g/L) •glucose 140
    •glucose 25 •K2HPO4 1.0
    •K2HPO4 1.5 •MgSO4 0.6 Parameter yang diamati :
    •MgSO4 0.6 •MnSO4 0.002 •OD
    •MnSO4 0.005 •FeSO4 0.002 •Glutamat conc
    •FeSO4 0.005 •thiamine 5.0 × 10−5 •RQ
    •ORP
    •corn slurry 25 •corn slurry 15 •REA
    •urea 2.5 (separately sterilized). •urea 3.0 (separately sterilized).
    Fermentasi batch normal dan abnormal
    Kesimpulan
    TERIMA KASIH
    Referensi
    Referensi

    You might also like