BIOMEDIC I

STRUCTURE AND FUNCTION OF MEMBRANS

AND EXTRACELLULAR MATRIX

Syahrijuita

BIOCHEMISTRY DEPARTMENT
Faculty of Medicine Hasanuddin University
2014
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 TOPIC:

 STRUCTURE AND FUNCTION OF

1.MEMBRANS

2. EXTRACELLULAR MATRIX

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 Learning Objectives

• Describe structure, function of

MEMBRANS

• Describe structure, function of

EXTRACELLULAR MATRIX

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MEMBRANS

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 EXTRACELLULAR MATRIX
• A complex network of secreted macromolecules
located in extra cellular space
• Associated chronic diseases such as: arthritis,
ateroclerosis, cancer and fibrosis
• Including:
-Collagens: provide tensile strength connect the
different constituents of the ECM
-Adhesive proteins: elastin, laminin,fibronectin
-Proteoglicans:space filling

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 Functions ECM
• Provides mechanical contact between cells
• Forms structures with spesial mechanical
properties : bone, cartilage, tendons and joints
• Filtering properties of the kidneys
• Insulated cells and tissue from one another
• Guides cells by forming pathway embryonic
development
• Cosmetic industry:ageing

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 COLLAGENS
Major and primery structural in the ECM,>30%
total protein in the body
Morethan 20 different types
Tensile, strength and flexibility
Triple helical structure (formed by folding of 3
peptide chains)
Chains size : 600 – 3000 amino acid
Repeating sequence of collagens:(Gly – X – Y)n
Synthesis in RER, Golgi Apparatus, ECM

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Collagens

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 Functions of Collagens:

1. Numerous hereditary genetic defect

2. Impact on the scleton, joints,
tendons,skins, eyes,etc
3. Disturbances in collagens synthesis
exp:loss of teeth, bleeding,skin damage
Vit C deficiency( Scurvy)

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HEREDITARY GENETIC DEFECTS OF COLLAGENS

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 ELASTIN

• Flexibility required for functions of :blood

vessels, lungs, ligament,skin, ECM
• The predominant protein of elastic fibers
• Only one gene
• A polypeptide 750 amno acid
• Tropoelastin  RER

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The Difference Collagens and Elastin

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 FIBRILIN
Protein (glicoprotein) = 350 kDa ;
imunohistokimia
Sindrom Marfan:
• mutasi gen (cromosom 15) for fibrilin;
dominan autosomal
• Lens Dislocation (ectopia lentis)
• hiperextensibilitas of joints; aracnodactili
(kerangka dan jari-jari panjang); tunica
media aorta weakness (dilatation of aorta
ascendens)
Mutasi gen (cromosom 5) ; aracnodactili
congenital contractur

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SCEMATIC FIBRONECTIN
(7 Domain Fungsional)

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 LAMININS

• Laminins are a family of noncollagenous in

basement membranes.
• Heterotrimeric molecules,large 850 kDa
• Interact with cells through multiple binding sites
• Has a central role in formation of crosslinks laminin and
type IV collagens

ENTAKTIN
• Binding at laminin
• Glikoprotein mengandung RGD
• Important at glomerulus filtration
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 PROTEOGLICANS

• are gel-forming components of the ECM

• Composeof peptide chains containing
covalently bound sugars
• Have a repeating disaccharide unit, an amino sugar and
a uronic acid:GLICOAMINOGLICANS
• Sindekan : Betaglikan; serglisin; perlekan,
Agrekan (tipe utama kartilago); versikan,
dekorin; biglikan; fibromodulin.

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TERIMA KASIH

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