Blood Film Interpretation

Drh. Ayu Joesoef, MSi

Outline

01 Blood film interpretation

02 Diagnostic approach to anemia

03 Modern PowerPoint Presentation designed

04 Modern PowerPoint Presentation designed

05 Modern PowerPoint Presentation designed

Blood film interpretation
 Polikromasia dan Hipokromasia

Polychromatophilic cells are young erythrocytes that have been released early. Usually, they are large and more
blue in color than mature erythrocytes. The blue color results from organelles (i.e., ribosomes, mitochondria) that
are still present in the immature cells. The presence or absence of polychromatophilic rbc is very important when
determining the cause of anemia. If immature cells are released, the likely cause of the anemia is blood loss or
blood destruction, with the bone marrow attempting to compensate by the early release of cells
Macrocytic erythrocytes

Large and have an increased MCV. The most common cause : increased numbers
of immature
erythrocytes that are polychromatophilic on Wright-stained blood films
Microcytic erythrocytes
Schistocytes Keratocytes
 Acanthocytes
Erythrocytes with irregularly spaced, variably sized spicules are called acanthocytes or spur cells (Figs. IIH, IIl).
Acanthocytes form when rbc membranes contain excess cholesterol compared to phospholipids. Alterations in rbc m
embrane lipids can result from increased blood cholesterol content or due to the presence of abnormal plasma lipoprotein composition
Echinocytes
Spherocytes
Eccentrocytes
Leptocytes
Stomatocytes
STRUCTURES IN OR ON ERYTHROCYTES
Heinz body
Basophilic stippling
Nucleated erythrocytes
Howell-Jolly bodies
Siderotic granules
Parasites
Hemobartonella felis
Blood film from an anemic cat. Note the numerous Hemobartonella felis organisms. Some of
these appear as small, ring-shaped organisms on the surface of a “ghost” erythrocyte that
has lysed (arrowhead). Others appear as rod-shaped structures on the edge of erythrocytes
(arrows). Insets. Higher magnification of both the ring and the rod-shaped forms. Wright
stain.
Cytauxzoon organisms
Babesia
Blood film from dogs with babesiosis. Left. Babesia canis organisms
appear as poorly staining, teardrop–shaped structures (arrowheads).
Right. Blood film from a dog with Babesia gibsoni (arrows). Wright stain.
Anaplasma
Viral inclusions
Erythrocyte arrangement on blood films
Rouleaux formation
rouleaux formation is normal in horses, and a slight amount also is normal in dogs and cats.
Rouleaux formation is enhanced, however, when the concentration of plasma proteins such
as fibrinogen or immunoglobulins is increased. Increased rouleaux formation often is
suggestive of a gammopathy; animals with multiple myeloma almost always have increased
rouleaux formation.
Agglutination