C-reactive protein

(CRP)
 C-reactive protein (CRP)

is a blood test marker for inflammation in the

body.
CRPis in the liver and its level
produced by testing
measured is the blood.
CRP is classified as an acute phase reactant,
which means that its levels will rise in response
to inflammation.
• Measures of CRP add to the
diagnostic in selected
procedur cases
differentiation (e-g.a in
between the and a viral
bacterial
einfection).
• An extremely elevated CRP is suggestive of a
possible bacterial infection.
• The CRP level may be useful also for
monitoring the effect of treatment and for
early detection of postoperative
complications or intercurrent infections.
 Tests for CRP

• Rapid latex agglutination test

• Principle:
– The test is based on the reaction between
patient serum containing CRP as the
antigen & theantibody coated
corresponding to treate
the surface of latex particle.The d
enhance
coated the detection of an agglutinate reaction
particle
when antigen is present in serum
s
the tested. being
 Tests for CRP

• Specimen- Serum Quality control

Include positive & negative
control serum.
• Reagent & materials
required
– CRP latex reagent
– Glycine – saline buffer
– Capillary pipette
– Applicator sticks
– Glass slide
– Serologic pipettes & rubber
bulb
 PROCEDURE
A.Qualitative
Test:
• Bring and specimens to
reagents
 temperature before use. room
 Place one drop (40 μl) of CRP Positive Control on field #1 of the
reaction slide. Place one drop (40 μl) of the CRP Negative Control
on field #2.

• Using a serological pipette place (40μl)

of undiluted test sample to field #3.
 PROCEDURE
A.Qualitative Test:
•Gently resuspend the CRP LatexReagent
and add one drop to each test field.
• Mix well with the provided stirring sticks.
•Rotate the slide for 2 minutes rea
and immediately under an oblique indirect d
light.
 INTERPRETATION

A.Qualitative Test:
• A negative reaction is indicated by a uniform
milky suspension with no agglutination as
observed with the CRP Negative Control.
• A positive reaction is indicated by any
observable agglutination in the reaction
mixture. The specimen reaction should be
compared to the CRP Negative Control
 INTERFERENCES

NON INTERFERING
SUBSTANCES:
• Hemoglobin (10g/dl)
• Bilirubin(20mg/dl)
• Lipemia(10g/dl)
 NOTE

• High CRP concentration samples may give

negative results .Re – test the sample again using
a drop of 20μl .
• The strength of agglutination is not indicative
of the CRP concentration in the samples
tested.
• Clinical diagnosis should not be made on
findings of a single test result, but should
integrate both clinical an laboratory data.
 LIMITATIONS

• Reaction time is critical. If reaction time

exceeds two minutes, drying of the reaction
mixture may cause false positive results.
• Freezing the CRP Latex Reagent will result in
spontaneous agglutination. phenomenon
(antigen excess).
• It is recommended, therefore, to check all
negative sera by retesting at a 1:10 dilution
with Saline Solution.