Urinary System Prelab

21 Feb 2014

1
 Squirrel Monkey Kidney (similar to one in the digital set)
B A

Arcuate Vessels

Capsule

Outer Medulla
Zone
Cortex Inner Zone
Calyx

Rectangles A and B indicate the regions pictured in the next two slides 2
 Squirrel Monkey Kidney Capsule and Cortex

Capsule

Circles indicate renal corpuscles consisting

of glomeruli and Bowman’s capsules.
These glomeruli belong to
cortical nephrons.

3
The cortex is a good place to find sections of PCT, DCT, and cortical collecting tubules/ducts
 Squirrel Monkey Kidney Medullary Rays and
Renal Corpuscles

The yellow lines roughly demarcate medullary rays. They include collecting tubules/ducts
and straight portions of proximal and distal tubules. A medullary ray and the tubules and
Bowman’s capsules that drain into it constitute a renal lobule.
 Squirrel Monkey Kidney
Renal Corpuscle, Vascular Pole with Macula Densa

3 PARTS OF BOWMAN’S CAPSULE

Vascular Pole

Glomerular
Capillaries
with Endothelium.
1. Bowman’s Space

2. Podocytes 3. Parietal layer

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 Squirrel Monkey Kidney
Renal Corpuscle, Vascular Pole with Macula Densa
Extraglomerular
Mesangial Cells

DCT
Macula Densa

PCT
DCT

Intraglomerular
Mesangial Cells?

6
 Squirrel Monkey Kidney
Renal Corpuscle with Urinary Pole

Urinary Pole

Beginning of PCT

Why is it rare to see both a urinary pole and a vascular pole in

the same slice of a renal corpuscle? 7
 DCT

PCT

This micrograph is not from a slide in your collection, but illustrates tubule varieties superbly. Note
dark and light cells in the cortical collecting duct, apical nuclei in the distal collecting tubule and
8
brush border and pinocytotic vesicles in the proximal convoluted tubule. Click to see the labels.
Squirrel Monkey Kidney Medullary Papilla with
Thin Limbs, Collecting Ducts and Vasa Recta

CD

CD CD
CD

Epithelium of papilla of
medullary pyramid. Transitional?

Calyx lumen

You are not expected to distinguish thin limbs from vasa recta. Collecting ducts 9
have the thickest epithelium of the three.
 Use this diagram to learn which
components of the nephron
are found in each part of the kidney
Cortex

Outer Stripe Outer Zone

Outer Zone
Inner Stripe Outer Zone

Medulla

Inner Zone

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 Rat kidney section(unilobar)digital Slide =Kidney100X

C
Inner
Stripe
-OZ Inner Zone

Estimate the boundaries of cortex and medulla. Within medulla estimate the boundaries
of the inner and outer zones and the inner and outer stripes of the outer zone. Click
11
to
see if you are right. Which segments of nephron are found in each area?
 Vasa Recta

Cortex Outer Stripe

Outer Zone
Inner Stripe Oute

AV
AA

Why does the inner stripe appear to have subdivisions? 12

 Comparison of different slides
Rat kidney (D3) Kidney, Monkey (D4)

D6

D4 and D6 are transverse sections. D6 is

stained with toluidine blue. The asterisks are in the
lumens of the calyces in D3 and D4. When examining
D3 begin at the tip of the papilla and work toward the
capsule. D6 has nice thin/thick descending limb transitions
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 Ureter of Rhesus Monkey

Adventitia

Oval outlines boundary between mucosa and muscularis. 14

 Ureter of Rhesus Monkey

Boundary between
circular and outer
longitudinal muscle layers?

Longitudinal muscle layer

Transitional Epithelium Mucosal connective

tissue

15
Human Bladder

16
 Human Bladder

Dome Cells

Dense irregular CT

17
Why is the plasma membrane of dome cells so thick?
The following three slides are not from your set. They are
Prelabeled and pretty. Enjoy.

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 JGA
MD
DCT

PCT

EGMs

JG Cells

CD

19
Slide is from BU web site
 MD

JGC

M
C

E
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Slide from collection of Dr. Ruth Bulger
Collecting Duct

MINOR
CALCYX
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