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The Effect of PH On The in Vitro
The Effect of PH On The in Vitro
pH of 8% sucrose buffer
1:1 Boric acid 2:1 Boric acid
1 2 3 4 5 mean 1 2 3 4 5 mean
6.4 90 80 85 95 90 88 80 80 90 80 90 84
5.6 70 65 75 70 70 70 60 75 70 65 60 66
4.8 0 0 10 0 15 5 0 0 0 5 0 1
4 0 0 0 0 0 0 0 0 0 0 0 0
Graphical Representation
pH v/s %growth
4 4.8 5.6 6.4
7
100 93
84
66 88 96
%GROWTH
80
70
60
40 0
1
20
0 5
0
2:1 boric acid
100
96
93
88
84
80
% GROWTH
70
66
60
40
20
5
0 0 1
4 4.8 5.6 6.4 7
1:1 boric acid 0 5 70 88 96
2:1 boric acid 0 1 66 84 93
pH
1:1 boric acid 2:1 boric acid
Observations
• The growth of pollen tubes were clearly seen using different microscopy techniques such as
dark field microscopy.
• Lysed cells showed the dispersal of cell matter around the pollen grain which too was clearly
visible.
• A large change in the germination of the pollen tube due to change in pH was seen.
• Boric acid in large amounts seemed to cause lysing of the pollen but helped in germination of
the pollen tube at a much faster and larger rate.
Different microscopic views of pollen, Pollen tube
germination and lysed pollen
Conclusion
• Pollen from both the flowers has a very low tolerance for the change in
pH.
• Pollen tube germination is highly dependent on external factors and
conditions and the amount of Boric acid present decides the rate at which
the pollen tube germinate thus proving that boron is highly essential and
that at different pH there is a visible difference in growth of pollen tube in
the presence of Boric acid.