Recent Advances of TDDS: The

Ultra-Deformable Vesicle (UDV)

BY SARAH ADINDA PUTERI
THE BACKGROUND
Why TTDS is potential to be one of the best way for drug delivery?

It has lots of
advantages Convenient, self- Non-invasive Minimizes the first-
medication is possible pass metabolism

But, the Stratum

Corneum as the
barrier is the
major problem
THE VESICULAR SYSTEM
Structure of vesicular system

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THE VESICULAR SYSTEM
Vesicular system comparation

Vesicular System Advantages Limitation(s)

Liposome Biocompatible, biodegradable Stability issue, less
penetration property
Transfersome Ultradeformable with high stability, high Difficulty in loading
penetration property, biodegradable, hydrophobic drugs
bio-compatible, incorporate low and
high molecular weight drugs, penetrate
deeper skin regions
Niosome Improved stability due to use of non- Skin penetration is not much
ionic surfactants effective
Ethosome Increased stability, increased skin Loss of product during
permeability, and inexpensive to transfer form organic to
formulate water media
THE UDV
Transfersome: a novel techniques for TDDS

Main Components:
1. An amphipathic ingredient
(phosphatidylcholine),
aqueous solvents self-
assemble into lipid bilayer
2. A bilayer softening
component (such as a
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biocompatible
oramphiphile drug): increases
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lipid bilayer
text here. flexibility and
permeability
THE UDV
Transfersome: a novel techniques for TDDS

Main Components:
1. An amphipathic ingredient
(phosphatidylcholine),
aqueous solvents self-
assemble into lipid bilayer
2. A bilayer softening
component (such as a
Sample Text surfactant
biocompatible
oramphiphile drug): increases
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lipid bilayer
text here. flexibility and
permeability
Strategy to Minimize the Limitations
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Optimization of formulation
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METHODS FOR TRANSFERSOME
PREPARATION

Vortexing-sonication method
01
mixed lipids (i.e. phospha-tidylcholine, EA, and the therapeutic agent) are
blended in a phosphate buffer and vortexed to attain a milky suspension. The
suspension is sonicated, followed by extrusion through poly-carbonate
membranes.

Suspension homogenization process

02
prepared by mixing an ethanolic soybean phosphatidylcholine solution with
an appropriate amount of edge-active molecule (sodium cholate). This
prepared suspension is subsequently mixed with Triethanolamine-HCl buffer to
yield a total lipid concentration. The resulting suspension is sonicated, frozen,
and thawed for 2 to 3 times.
METHODS FOR TRANSFERSOME
PREPARATION

Modified handshaking process

03
drug, lecithin (PC) and edge activator were dissolved in ethanol: chloroform
(1:1) mixture. Organic solvent was removed by evaporation while hand
shaking above lipid transition temperature (43°C). A thin lipid film was formed
inside the flask wall with rotation. The thin film was kept overnight for complete
evaporation of solvent. The film was then hydrated with phosphate buffer (pH
7.4) with gentle shaking for 15 minute at corresponding temperature.

Aqueous lipid suspension process

04
drug-to-lipid ratio in the vehicles is fixed between 1/4 and 1/9. Vesicles with the
size ranging from 100-200 nm are prepared by using soyphosphatidylcholine
with the standard deviation of size distribution (around 30%). This formulation
could be prepared by suspending the lipids in an aqueous phase wherein the
drug is dissolved.
METHODS FOR TRANSFERSOME
PREPARATION

Centrifugation process
05
phospholipids, surfactants and the drug are dissolved in alcohol. Then the
solvent is removed by rotary evaporation under reduced pressure at 40oC.
Final traces of solvent are removed under vacuum. Then the deposited lipid
film is hydrated with the appropriate buffer by centrifuging at 60 rpm for 1 hour
at room temperature. At room temperature, the resulting vesicles are swollen
for 2 hours. The multi-lamellar lipid vesicles obtained which are further
sonicated at room temperature.
THE UDV
Application of Transfersome

Delivery
of Insulin

Delivery of Delivery of
herbal drugs 01 corticosteroids

TRANSFERSOME
Delivery of
Delivery of
proteins and
anesthetics
peptides
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This is a of
Delivery sample text.
Delivery of
anticancer Insert your desired
interferon
drugs (INF)text here.
THE UDV
Application of Transfersome

Delivery of
Insulin

Delivery of
herbal drugs 01
Delivery of
corticosteroids
01

TRANSFERSOME Delivery of Insulin

Delivery of
Delivery of
proteins and Conventional insulin:
anesthetics
peptides inconvenient. After
application of
transfersulin on the intact
Delivery of Delivery of
skin, therapeutic effect
anticancer interferon
drugs (INF) observed after 90-180 min
THE UDV
Application of Transfersome

Delivery of
Insulin

Delivery of Delivery of
corticosteroids
02
herbal drugs 01

Delivery of
TRANSFERSOME
Delivery of
corticosteroids
Delivery of
proteins and
anesthetics
peptides
Transfersome
encapsulation helps
optimization of
Delivery of Delivery of corticosteroid delivery
anticancer interferon into skin.
drugs (INF)
THE UDV
Application of Transfersome

Delivery of
Insulin

Delivery of Delivery of
corticosteroids
03
herbal drugs 01

Delivery of proteins
TRANSFERSOME
Delivery of
and peptides
Delivery of
proteins and
anesthetics Transfersome (the adjuvant
peptides
immunogenic serum
albumin) is somewhat
Delivery of Delivery of identical to subcutaneous
anticancer interferon injection.
drugs (INF)
THE UDV
Application of Transfersome

Delivery
of Insulin

Delivery of Delivery of
corticosteroids
04
03
herbal drugs 01

Delivery of
Delivery of proteins
TRANSFERSOME
Delivery of interferon (INF)
and ofpeptides
Delivery
proteins and
anesthetics Hafer et al. studied the
peptides
formulation of
transfersome containing
Delivery of Delivery of interleukin-2 (IL-2) and
anticancer interferon INF-α
drugs (INF)
THE UDV
Application of Transfersome

Delivery
of Insulin

Delivery of
herbal drugs
Delivery of
corticosteroids
05
04
03
01

Delivery of
TRANSFERSOME
Delivery of anticancer drugs
Delivery of
proteins and
anesthetics Result found to be
peptides
Sample Text favorable when
methotrexate was tried
This is a of
Delivery sample text. for transdermal delivery
Delivery of
anticancer Insert your desired
interferon using transfersome
drugs (INF)text here. technology.
THE UDV
Application of Transfersome

Delivery
of Insulin

Delivery of Delivery of
corticosteroids
04
06
03
herbal drugs 01

Delivery of
TRANSFERSOME
Delivery of anesthetics
Delivery of
proteins and
anesthetics Application of
peptides
transfersome containing
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anesthetics induces a
Delivery of
This is a of
Delivery sample text. topical anesthesia, under
anticancer Insert your desired
interferon suitable conditions, within
drugs (INF)text here. 10 min. The effect: 80%
THE UDV
Application of Transfersome

Delivery
of Insulin

Delivery of
herbal drugs
Delivery of
corticosteroids
03
07
01

Delivery of herbal
TRANSFERSOME
Delivery of drugs
Delivery of
proteins and
anesthetics Xiao-Ying et al. showed
peptides
the better topical
Sample Text
absorption of
Delivery of
This is a of
Delivery sample text. transfersomes of
anticancer Insert your desired
interferon capsaicin in comparison
drugs (INF)text here. to pure capsaicin
Conclusion
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References
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THANK YOU.