RECIRCULATING AQUAPONICS

PRODUCTION OF LETTUCE
(Lactuca sativa), PECHAY (Brassica
rapa), AND MUSTASA (Brassica
juncea)
WITH RED TILAPIA

Keanno Rod M. Peliña1

Samuel B. Letana1
Ma. Rhazel M. Sevilla1
Roberto J. Lacson2

1Bachelor of Secondary Education IV (Biological Science), CvSU Naic, Bucana, Naic, Cavite
2Instructor III; Research Adviser, CvSU Naic, Bucana, Naic, Cavite
 INTRODUCTION
• Aquaponics is the integration of
aquaculture and hydroponics which deals
to a closed production system with soilless
culture.

https://www.eumofa.eu/data#aquacultureTab https://www.brandcrowd.com/gallery/brands/pictures/picture145808
65091860.jpg
 INTRODUCTION
• Aquaponics
systems consist of
network of pipes
and pumps that
circulate waste
water from a
freshwater fish tank
http://aquaponicsplan.com/wp-content/uploads/2013/04/aquaponics-
to water filled systems-6.jpg

garden beds.
 INTRODUCTION
• Advantage of using aquaponics system:
– no use of external nitrogen fertilization;
– complete reuse of water;
– better climatic control for fish growth than
pond systems;
– has a higher system resiliency due to
symbiotic presence of microorganism;
– it has a lower nutrients concentration rather
than hydroponics up to 10x efficiency of water
use.
 Statement of the Problem
• This study is design to determine the
production of three leafy plants in
recirculating aquaponics system.
 Statement of the Problem
Specifically, this sought to answer the
following questions:
1.What are the ranges of physico-chemical
water parameters in recirculating aquaponics
system tank in terms of:
– Ammonia (NH3) - pH
– Dissolved Oxygen (DO) level - Turbidity
– Nitrate (NO3) - Total Dissolved Solids (TDS)
– Nitrogen – Total
 Statement of the Problem
2. What are the growth rate of Pechay,
Mustasa and Lettuce in aquaponics
system?
3. What is the survival rate of Pechay,
Mustasa and Lettuce?
4. Is there significance differences in the
growth performance of:
– Pechay - Mustasa - Lettuce
 Objectives of the Study
1. Determine the range of physico-chemical water
parameters in recirculating aquaponics system
tank in terms of:
– Ammonia (NH3)
– Dissolved Oxygen (DO) level
– Nitrate (NO3)
– Nitrogen – Total
– pH
– Total Dissolved Solids (TDS)
– Turbidity
 Objectives of the Study
2. Determine the growth rate of Pechay,
Mustasa and Lettuce in aquaponics system?
3. Determine the survival rate of Pechay,
Mustasa and Lettuce?
4. Determine the significance differences in the
growth performance of:
– Pechay
– Mustasa
– Lettuce
Importance and Significance of the Study

The result of the study would benefit

the following sectors of the community.

• Community. This will serve as their basis/

guidelines in establishing their own system
at their backyard or garden for own
consumption or business purposes.
Importance and Significance of the Study

• Researchers. This will serve as reference

for future and related studies on
aquaponics system.
• Extentionists. The result could be used
for extension purposes.
Importance and Significance of the Study

• For fisheries technologists and

aquaculturist. For further development in
establishing aquaponics system, this study
can be used to support the result of their
study and project development.
Scope and Delimitation of the Study

• The experimental period took 30 days.

• Three treatments with nine replicates were
used.
• Pechay (Brassica rapa), Lettuce (Lactuca
sativa), and Black Mustard Seed or
Mustasa (Brassica juncea) and Red tilapia
were used in the study.
Scope and Delimitation of the Study

• One fish tank stocked with 300 red tilapia

#24 fingerlings was used.
• The experiment started from the planting
of pechay, mustasa and lettuce seeds until
they reached specific length in 30 days.
Scope and Delimitation of the Study

• Commercial feeds were used as food

of the fish.
• The pH, Turbidity, DO level, TDS,
Ammonia, Nitrogen – Total, Nitrate
was monitored and tested.
Scope and Delimitation of the Study

• The study focused on the growth

performance and survival rate of the
Lettuce (Lactuca sativa), Pechay (Brassica
rapa), and Black Mustard Seed or
Mustasa (Brassica juncea) with the use of
Red Tilapia.
• Specific growth rate and survival rate were
observed daily.
Scope and Delimitation of the Study

• Identification of the cause of mortality of

tilapia; inclusion of fertilizer as pond
preparation and the comparison of
commercial production of tilapia through
body weight, size, the growth rate and
survival rate of Red Tilapia were not
included in the study.
 Conceptual Framework

Input Process Output

• Recirculating • Monitoring water • The Suitable
Aquaponics parameters. plant for
System • Counting Recirculating
• Red Tilapia • Measurement Aquaponics
Fingerlings system
• Pechay • Data Analysis
(seeds) - Growth and
yield of:
• Mustasa • Vegetable
(seeds) crops
• Lettuce • Red Tilapia
(seeds)
 METHODOLOGY
• Study Area
(Geographical Location of CvSU Naic – Mabulo Annex)

https://www.google.com/maps/search/Cavite+State+University+-
+Naic+Mabulo+Annex/@14.3192679,120.7453805,1417m/data=!3m2!1e3!4b1
 METHODOLOGY
• Gathering of #24 fingerling red tilapia at
BFAR Region IV – A office
 METHODOLOGY
• Experimental Design. This study used
completely randomized design (CRD) as its
experimental design. Randomization using
simple random sampling (SRS) was applied
to determine the position of each replicates.
The study has 3 treatments and 9 replicates
with a total number of 27 experimental units.
Treatment 1 was the Lettuce Seeds while
Treatment 2 was the Pechay Seeds and the
Treatment 3 was the Mustasa Seeds.
 METHODOLOGY
• Lay-out of the experiment.
 METHODOLOGY
• Preparation of PVC Pipes and Tank.
 METHODOLOGY
• Recirculating aquaponics system
 METHODOLOGY
• Stocking the Fish.
 METHODOLOGY
• Growth Rate and Survival Rate of
Plants. Ruler was used to measure the
length (cm) of lettuce, pechay, and
mustasa daily to monitor growth rate.
• The survival rate of plants was computed
using the formula:
 METHODOLOGY
• Statistical Analysis. Data gathered on
the growth and survival rate of plants were
subjected to one-way ANOVA and Tukey
HSD and Duncan Post Hoc test.
 RESULTS
and
DISCUSSION
Physico-chemical parameters
Table 1. Test result of physico-chemical
parameters of water quality samples

AMMONIA as DISSOLVED NITRATE as TOTAL

TURBIDITY** TDS
NH4-N** OXYGEN** NO3-N** NITROGEN**
(mg/L) (mg/L)
(mg/L) (mg/L) (mg/L) (mg/L)

12 760 < 2.0 3.4 < 0.5 2.5

Survival Rate of Plants

TREATMENT
1 2 3
0% 67% 22%
Survival Rate of Plants
 Graphical representation of growth of three
vegetable plants for 30 days.
Legend: Treatment 1 - Lettuce Treatment 2 - Pechay Treatment 3 - Mustasa
Growth Rate and Performances

Mean SD
Treatment 1 – Lettuce 0.00 (±0.00)
Treatment 2 – Pechay 51.79a (±8.33)
Treatment 3 – Mustasa 26.34b (±5.23)
Note: Means within the column having a different superscript are significantly
different to each other. The mean difference is significant at the 0.05 level.

* Treatment 2 was the most produced vegetable plants among

the rest treatments.
* Treatment 3 - only 2 replicates was produced a proper
growth.
 CONCLUSION
• The level of ranges of physico-chemical
parameters is highly different for the
optimal water quality values for productive
and well performing Aquaponics Systems
for Tilapia.
• But in terms of pH, it was maintained by
the ideal scale of 7.0 which is similar to the
study of Sallenave (2016).
 CONCLUSION
• In terms of survival rate, pechay was
recorded the most survived plant at 67%.
• There is significant difference (p<0.05) in
terms of growth performance of pechay
and mustasa.
• Therefore, the Pechay was the most
appropriate vegetable plant in aquaponics
system.
 Recommendation
The researchers recommend further
study regarding the germination process of
each leafy plants specifically in Lettuce
seeds. Likewise, the researchers must use
the leafy plants with similar time of
germinating process and growth like
Mustasa and Pechay seeds.
 Recommendation
• Furthermore, it was also recommended to
build an experimental setup in ideal
environment with an ideal temperature of
25–30 C with a proper connection of hose
in each PVC pipe.
Thank you!