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Layer by Layer Immobilization of Amphoteric Carboxymethyl Chitosan Onto Biocompatible Silk Fibroin Nanofibrous Mats
Layer by Layer Immobilization of Amphoteric Carboxymethyl Chitosan Onto Biocompatible Silk Fibroin Nanofibrous Mats
Layer by Layer Immobilization of Amphoteric Carboxymethyl Chitosan Onto Biocompatible Silk Fibroin Nanofibrous Mats
Scanning Electrron
Microscopy (SEM)
Cell Proliferation and Toxicity test
Murine Mc3T3 pre-
esteoblast cells were
cultured
Scanning Electron
Microscopy
RESULTS AND DISCUSSION
Morphology Investigation
Fig. 1. FE-SEM images of electrospun SF nanofibrous mats (a) and SF mats after physical crosslinking (b).
Morphology Investigation
Fig. 2. FE-SEM images of LBL modified nanofibrous mats with different bilayers: LBL0.5 (a), LBL1 (b), LBL5
(c), LBL5.5 (d), LBL10 (e) and LBL10.5 (f).
Composition and
Morphology Investigation
Structural Analysis
Fig. 3. The FT-IR spectra of raw materials and various nanofibrous mats (a); the XPS narrow scans of LBL5.5
with the curve fit of C1s (b) and N1s (c).
Morphology Composition and TGA data and mechanical
Investigation Structural Analysis properties of samples
Fig. 4. The thermal properties (a) and mechanical behavior (b) of various mats.
Morphology Composition and TGA data and mechanical The Hydrophobicity of
Investigation Structural Analysis properties of samples our mats
Fig. 5. WCA data of SF and LBL modified nanofibrous mats over times.
Bacterial Inhibition
Activity
Fig. 6. Antibacterial activity of samples against E. coli and S. aureus. Error bars represented standard deviation
(n = 3).
Bacterial Inhibition
CCK-8 assay
Activity
Fig. 7. FE-SEM images of E. coli adhered on the SF mats and LBL modified mats: SF (a), LBL0.5 (b), LBL1 (c),
LBL5.5 (d), LBL10 (e), LBL10.5 (f).
Bacterial Inhibition
CCK-8 assay
Activity
Fig. 8. FE-SEM images of SF mats and LBL modified mats with MC3T3 cells seeded on the surface for 48 h (a–g: SF
(a), LBL0.5 (b), LBL1 (c), LBL5 (d), LBL5.5 (e), LBL10 (f), LBL10.5 (g)) and cell viability results after 24 h’s,
48 h’s and 72 h’s cultivation (h). Error bars represented standard deviation (n = 3).
Conclusions
Conclusions
• LBL modified nanofibrous mats exhibited higher thermostability and
stronger mechanical properties after coating with CMC
• The surface of mats became rough and the hydrophily of mats enhanced
after the LBL procedure.
• The antimicrobial property of LBL structured mats increased a lot with the
introduction of CMC on the surface of the nanofibrous mats.
MYRDomino