Genetic

Technologies:
Amplifying, Modifying, and
Monitoring DNA
Prepared by John Lloyd Jordan Fausto
Biotechnology
• Use or alteration of cells or biological
molecules for specific application
• Transgenic organisms are possible, but the
genetic code is universal
• Ethical and legal issues to be considered
including patent laws
Monitoring Gene Function
• Gene Expression Profiling
 Identifies all of the genes in a cell or tissue that
are making messenger RNA
 Indicates genes transcribed
 Used to find and diagnose a disease or condition
or to see how well the body responds to
treatment.
Monitoring Gene Function
• DNA Variation Screening
 The application of a test on people for the
systematic early detection or exclusion of a
hereditary disease, a genetic predisposition to a
disease, or to whether a person carries a
predisposition which may produce a hereditary
disease to their offspring
 Detects mutations in Single Gene Polymorphisms
(SNPs)
Monitoring Gene Function
• Microarray Comparative Genomic
Hybridization
 A new cytogenic technology that evaluates areas
of the human genome for gains or losses of
chromosome segments at a higher resolution than
traditional karyotyping
 Deletions and amplifications of DNA sequences
between cells or species
Monitoring Gene Function
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Monitoring Gene Function
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Monitoring Gene Function
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Monitoring Gene Function
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