Professional Documents
Culture Documents
For FK & FKG Usu Students Only: Histology Department
For FK & FKG Usu Students Only: Histology Department
For FK & FKG Usu Students Only: Histology Department
Prepared by:
1
LokDonLub
URINARY SYSTEM
• Consist of:1,2,3,4
1. A pair of KIDNEYS glandular component
2. A pair of URETERS
3. An URINARY BLADDER
4. An URETHRA
KIDNEY
• Functions :1,2,3,4
1. Clear blood plasma of metabolic wastes
(creatinine, uric acid, urea)
2. Regulate fluid osmolality & volume
3. Help maintain Acid-Base balance
4. Eliminate drugs, pesticides etc
5. Endocrine functions (renin & erythropoetin)
6. Activating inactive vitamin D to active form ( 1,25
dihydroxy vitamin D3)
MACROSCOPIC STRUCTURE
• Bean shaped with 2 layer of thin-strong conn tissue
capsule:1
– Outer fibrous layer: type I & type III collagen & occasional
fibroblasts
– The inner layer: types I & III collagen & myofibroblasts
• Hillum :
– A concave border, enter point of renal arteries & nerves
– Exit point of renal vein, lymphatic vessels & ureter
Expanded
upper end of
ureter
KIDNEYS PARENCHYMA
• Kidneys parenchyma divided into:
1. Cortex
• Outer region, darker & granular appearing
• Subdivided into: cortical labyrinth &
medullary rays
2. Medulla
• Paller & smoother
• Consist of medullary/renal pyramids
Cortical Labyrinth &
Medullary/Renal
Pyramids Medullary Rays
THE CORTEX
• The cortex Cortical labyrinth & 500 or so
medullary rays:
– The cortical labyrinth: composed of
• The renal corpuscles
• The convoluted tubular portions of the nephron
– Each medullary ray: an extension of the renal
medulla (pars recta of proximal & distal convoluted
& collecting ducts) into cortex forms the kidney
lobule
THE MEDULLA
• Composed of 10 to 18 renal pyramids each
constitute a lobe of the kidney
• Major Divisions:2
– Renal corpuscle
– Proximal convoluted tubule located primarily in cortex
– Thin & thick segment (loop of Henle) Descend into
medulla, then ascend back to cortex
– Distal convoluted tubule
Ascending thick
Nephron Loop / Pars
Recta distal tubule
Descending thick Nephron
Loop / Pars Recta Proximal
tubule
Collecting Tubule
RENAL LOBE THE
URINIFEROUS
TUBULE NEPHRON
TYPES
RENAL CORPUSCLE
• The beginning of a nephron, Ø ±200 µm, differ in
size:1,2
– Near medulla larger & tubules longer
– Periphery of cortex smaller & tubules shorter
• Consist of:1,2,3
– The Glomerular Capillaries: fenestrated, large pores (Ø
60-90 nm), lacking diaphragms. The endothelial cell
possess aquaporin-1 channels for rapid passage of water
– The Glomerular - Double-Walled Epithelial Capsule
(Bowman’s Capsule):
• Viceral/Internal Layer closely envelopes the capillaries
PODOCYTES
• Parietal Layer outer surface of capsule simple squamous
epithelium
VASCULAR POLE :
afferent & efferent
arterioles enter &
leave
URINARY/TUBULAR
POLE :
Region opposite
vascular pole, where
the proximal
convoluted tubule
begins
PODOCYTES
• Modification of viceral
epithelium cells → Speciallized,
large & stellate cells
• Contractile
• Respond to angiostensin II
mediate blood flow through
glomerular capillaries
MESANGIAL CELLS FUNCTIONS:1
PROXIMAL TUBULE
• Continous with parietal
Bowman’s capsule
• 2 Region:
– Convoluted portions/pars
convoluta
– Straight portions/pars recta
• CONVOLUTED PORTIONS:
– Most frequently seen in cortex
– Reabsorb 60–65% of water
filtered in renal corpuscle, also
almost all of nutrients, ions,
vitamins & small plasma
proteins
– Single layer of pyramidal cells:
• Acidophilic cytoplasm
numerous mitochondria
• Prominent microvillus
border/brush border for
reabsorption
• Not all show nucleus because
large size of cells
PROXIMAL CONVOLUTED TUBULE
• 3 regions:
– Descending thin limb
– Henle’s loop
– Ascending thin limb
• Function: autoregulation
of the glomerular filtration
rate (GFR) & controlling
blood pressure
• Juxtaglomerular Cell:
– Highly modified smooth
muscle in wall of afferent • Lacis Cells:
arteriole • Same supportive functions as inside
– Contain secretory granule the glomerulus
– Produce renin • Also transmit signals from macula
densa into the glomerulus, affecting
vasoconstriction
DISTAL TUBULE
• Convoluted Portions Of
Distal Tubule:
– Shorter than PCT fewer
seen
– Lumen wider than PCT
– Cells shorter & lighter
– Nuclear seen in each cell
– No brush border
– Few luminal microvilli
• Initial segment cortex
COLLECTING DUCT
• Cuboidal lining
epithelium consist of :
– Principal (light) cell
cuboidal, central nuclei,
light stain, possess a
single, nonmotile, apically
cilium (functions as a
mechanosensor that
monitors fluid flow)
– Intercalated cell fewer, Intercalated cells 2 types:
stain more deeply, •Type A cells secrete H+ into the
scattered between tubular lumen
principal cells •Type B cells resorb H++ & secrete
HCO3-
EXTRARENAL PASSAGES
• All have similar structure (mucosa, muscularis, adventitia) except
urethra4
• Consist of :
1. Minor & major calyces
2. Renal pelvis the expanded proximal end of the ureter & serves
to collect the urine excreted from the kidney & to transport it to
the ureter proper
3. Ureter Epithelial lined muscular tubes designed to transport
urine from the kidneys to urinary bladder with aid of peristalsis
4. Urinary bladder:
• An epithelial-lined muscular viscus has ability to distend &
accommodate up to 400 - 500 mL of urine without a change in
intraluminal pressure
• Able to initiate & sustain a contraction until the organ is empty
5. Urethra
Microscopic of Calyces, Renal Pelvis,
Ureters & Bladder
• The urinary bladder, ureter, & renal pelvis
have a similar anatomic composition:
– The innermost layer transtitional epithelium
– A lamina propria
– Muscularis propria smooth muscle
– Adventitia fibroelastic tissue
• 3 regions:
– The superficial cells in contact with the urinary space
– The intermediate cells
– The basal cells lie on a basement membrane
Normal urothelium. The mucosa may be up to seven cells
thick in the bladder, but thickness will vary as a consequence
of distension and other factors. The superficial (umbrella)
cells have ample eosinophilic cytoplasm
Normal bladder is composed of urothelium, lamina propria
with mediumsized vessels, & thick muscle bundles of
muscularis propria.
URETER
Lamina Propria
Lamina Propria
• Lies between mucosal basement membrane &
muscularis propria
• Sexually dimorphic:
– Males → terminal duct for both urinary & genital
systems
– Females → urinary system only