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  • Module 1 - Animal Handling Basic Information of Pharmacology Practicum

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    madeline
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    1) This document provides information about in vitro and in vivo experiments that will be conducted as part of an animal handling and pharmacology practicum. 2) For in vitro experiments, students will study cell structure, reproduction, diffusion, osmosis, and tonicity by observing dye diffusion through various mediums and measuring solution weights over time. 3) For in vivo experiments, students will consider factors influencing experimental results, anatomy, animal handling, drug administration, surgery, and animal identification techniques before conducting experiments on rats and mice.

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    ANIMAL

    Original Title

    Module 1_Animal Handling Basic Information of Pharmacology Practicum (1)

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    1) This document provides information about in vitro and in vivo experiments that will be conducted as part of an animal handling and pharmacology practicum. 2) For in vitro experiments, students will study cell structure, reproduction, diffusion, osmosis, and tonicity by observing dye diffusion through various mediums and measuring solution weights over time. 3) For in vivo experiments, students will consider factors influencing experimental results, anatomy, animal handling, drug administration, surgery, and animal identification techniques before conducting experiments on rats and mice.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pptx, pdf, or txt
    21 views14 pages

    Module 1 - Animal Handling Basic Information of Pharmacology Practicum

    Uploaded by

    madeline
    1) This document provides information about in vitro and in vivo experiments that will be conducted as part of an animal handling and pharmacology practicum. 2) For in vitro experiments, students will study cell structure, reproduction, diffusion, osmosis, and tonicity by observing dye diffusion through various mediums and measuring solution weights over time. 3) For in vivo experiments, students will consider factors influencing experimental results, anatomy, animal handling, drug administration, surgery, and animal identification techniques before conducting experiments on rats and mice.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    of 14

    MODULE 01

    ANIMAL HANDLING & BASIC


    INFORMATION PHARMACOLOGY
    PRACTICUM
    In Vitro In Vivo
    Experiments Experiments
    In Vitro Experiments
    In Vitro Experiments (In practicum)
    A. Cell Structure and the B. Cell activity
    reproduction process

    Please fill and complete picture in Diffusion Osmosis


    the module (Preliminary Task) !!

    Tonicity
    Each group will do all cell activity
    experiments
    Cell activity
    • Simple Diffusion : using KMNO4 and see how KMNO4
    spread in the water (record time)
    • Agar Diffuson : KMNO4 spread in the agar (record
    Diffusion
    time)
    • Diffusion through membrane : using chellophane
    membrane

    • Record the weight of solution in cellophane every 15


    Osmosis
    minutes

    • Determine type of solution : hypotonic, isotonic,


    Tonicity
    hypotonic
    In Vivo Experiments
    Factor-factor which may influence in vivo
    experimental result
    Internal factors External factors
    • Age • Oxygen supply
    • Sex • Environmental maintenance,
    • Genetic features such as : cage condition, novelty,
    number of animal, animal house
    • Health status condition
    • Nutritional state • Structural maintenance
    • Body weight
    • Body surface
    In Vivo experiments (In Practicum)

    Anatomi Animal
    • Body Cavity • Handling
    • Anatomical Regions • Drug administration
    • Anatomical Terminology • Surgery
    • Topographic Anatomy (Rats) • Animal Identification

    Please fill and complete picture


    in the module (Preliminary Task)
    !!
    Animal

    Handling Drug Administration

    • Participants will try to • Route : oral, intraperitoneal,


    handling mouse and rats  subcutaneous
    assistance will assess • Participants should do oral
    • Another animal will be route mouse and rats
    demonstrated by assistance • If participants can do
    • Sucrificing animal will be another route will get
    demonstrated by assistance addiotional point
    Animal (Surgery)
    • Participants will do surgery Group Animal Sex

    together in group 1
    Rat
    Male
    2 Female
    • Take picture and share to 3 Male
    another group in a shift 4
    Mouse
    Female
    • Identify animal organ 5 Rat Male
    6 Mouse Female
    Animal Identification

    Picrat identification Identification by location


    Location Identification
    Head H
    Back B
    Tail T
    Head back HB
    Head tail HT
    Back tail BT
    Head back tail HBT
    Fore leg FL
    Hind leg HL
    legs LS
    Right fore leg RFL
    Left fore leg LFL
    Left hind leg LHL
    2 right legs 2 RL
    2 left legs 2 LL
    Blank B
    Thank you

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