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4 S rule

• Specific: use approach that specifically target the cells/molecules of interest


rather than a global approach targeting more than one cell/molecule

• Stick to what you know: use the paper analyzed in class and assembled toolboxes
to determine your gap in knowledge and always base your design on known design
found in the literature

• Simple: use the simple rather than complicated approach

• Succinct: try to explain everything without diluting your message


Ask yourself, “do I need that”?
What to do

• Evaluate the question and decide on a general approach

• What do you know about the topic/methodology that was


discussed in the paper or previous papers/toolboxes?

• What do you need to research?

• Be very specific when searching

• Start researching with a review paper then go to cited


paper to extract methodological details

• Current protocols in Immunology

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