Water Temperature as a

Limiting Factor to Guppy’s

Survival
CONTROL
OBSERVATIO 5 min. 10 min. 15 min. 20 min. 25 min. 30 min.
NS
No. Of 340 520 320 334 352 332
opercular
movement
Position in Center, near Side of the Near bottom Bottom Near bottom Near bottom
the beaker bottom beaker
Distribution Scattered Scattered In groups Scattered Inch near 1 inch near
each other each other
Swimming Very fast Moderate Slow Fast Fast Moderate
speed
Number of 0 0 0 0 0 0
deaths
@10° Celsius Water temp.
OBSERVATIO 5 min. 10 min. 15 min. 20 min. 25 min. 30 min.
NS
No. Of 320 315 265 245 230 216
opercular
movement

Position in Center, near Side of the Near bottom Bottom Near bottom Near bottom
the beaker bottom beaker

Distribution Scattered Scattered In groups Scattered Inch near 1 inch near

each other each other

Swimming Very fast Moderate Slow Slow Slow Slow

speed
Number of 0 0 1 2 3 5
deaths
@ 32° Celsius
OBSERVATION 5 min. 10 min. 15 min. 20 min. 25 min. 30 min.
S

No. Of 330 367 351 370 361 334

opercular
movement

Position in Center, near Center of the Near the Near the Near the Near the
the beaker bottom beaker surface surface surface surface
Distribution Scattered Scattered In groups Scattered Inch near inch near
each other each other
Swimming Very fast Moderate Moderate Moderate Moderate Moderate
speed
Number of 0 0 0 0 0 0
deaths
Conclusion
 Guppy fish will tolerate water at up to 90 °F (32 °C), but never for a
prolonged time and only if the water is well aerated because the
warmer the water, the lower the oxygen level in it.
 Their ideal temperature range is set between 72-82 °F (22-28 °C).
That said, guppy fish can survive in lower temperature water that
goes as low as 60 °F (15 °C)
 Trial 1
Salinity Treatments
Control A B C D

Root Shoot Root Shoot Root Shoot Root Shoot Root Shoot
Seed length
(mm)
length(
mm)
length
(mm)
length(
mm)
length
(mm)
length(
mm)
length
(mm)
length(
mm)
length
(mm)
length(m
m)

1 9.2 96 8.7 90.6 5.3 56 5.2 48 3.2 36

2 11.3 100.6 9.2 102.3 6.2 50 0 0 0 0
3 9.4 98 8 92 4.3 53 4.3 47.2 3.6 38.3
4 8 86 5.6 72 6.3 60 6 50 0 0
5 8.9 89 4.2 72 7 70.3 5 45 4.1 39.2
6 9.1 93.2 6.9 88 6 59.2 0 0 3 30
7 7.2 72.3 9 98 8.6 76.2 6.1 53.2 0 0
8 6.3 63.2 6.5 87.2 5 73.5 7.3 59.1 2.9 27.6
9 6.11 63 10.3 111.3 3 72.2 8.2 62 0 0
10 6.2 69 7 90.2 9.6 76 3.5 43.4 2.6 26.2
Average 8.17 84.03 7.54 80.31 6.13 64.81 4.56 40.79 1.94 19.73
R/S ratio 0.097 0.094 0.092 0.111 0.098
Germination 100% 100% 60% 40% 40%
 Trial 2
Salinity Treatments
Control A B C D

Seed Root Shoot Root Shoot Root Shoot Root Shoot Root Shoot
length length( length length( length length( length length( length length(m
(mm) mm) (mm) mm) (mm) mm) (mm) mm) (mm) m)

1 23 126.5 16.3 87 14 63 0 0 0 0
2 26 128.5 13.6 79 0 0 11 46 0 0
3 28.9 125.8 15.3 85 10.6 57.6 9 45.3 0 0
4 30 120 16.3 82 12 63.5 0 0 10.6 20.6
5 34 119.2 20 100 0 0 10 44 0 0
6 40 110 19 98 10 57.2 0 0 13 25.2
7 36 115 17.6 86 0 0 12.1 47.1 12.1 21.2
8 38 120.3 13.3 72 11 58 0 0 9.8 29.7
9 37 116 10 70 0 0 0 0 0 0
10 35 117.2 18.9 97 13 64 0 0 0 0
Average 32.5 119.7 15.94 85.6 7.06 36.33 4.2 18.53 4.55 9.67
R/S ratio 0.272 0.186 0.194 0.226 14.22
Germination 100% 100% 60% 40% 40%
 Trial 3
Salinity Treatments
Control A B C D

Seed Root Shoot Root Shoot Root Shoot Root Shoot Root Shoot
length length( length length( length length( length length(m length length(m
(mm) mm) (mm) mm) (mm) mm) (mm) m) (mm) m)

1 27 120.9 23 100.3 21 85 0 0 0 0
2 26.2 119 20 98.2 0 0 19 76.2 0 0
3 28.4 126 26 120 19 76 0 0 0 0
4 30.2 118.3 25.2 118 20 78.6 0 0 15.4 60.3
5 36 109.2 0 0 23.5 80.2 18 73 0 0
6 32 115.6 20 92 22 74.3 21 78.3 0 0
7 29 117.3 22.1 100.9 0 0 20 79 50.6 56.5
8 26 112.2 23.6 106.1 21.5 72.6 19.6 78.2 0 0
9 24 109.3 25 110.3 0 0 18.2 75.6 13.5 58.2
10 25 120 0 0 18 70.2 0 0 0 0
Average 28.38 116.76 18.49 85.27 14.5 53.69 11.58 43.76 2.74 17.47
R/S ratio 0.243 0.217 0.270 0.265 0.154
Germination 100% 80% 70% 60% 30%
 Trial 1 control
120

100

80

60

40

20

0
1 2 3 4 5 6 7 8 9 10

Root length (mm) Shoot length (mm)

 Trial 1 A
Trial 1 B
120
90
100 80
70
80
60
60 50
40
40 30
20
20
10
0 0
1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 11

Root length (mm) Shoot length (mm) Root length (mm) Shoot length (mm)

Trial 1 C Trial 1 D
70 45

60 40
35
50
30
40 25
30 20
15
20
10
10 5
0 0
1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 11

Root length (mm) Shoot length (mm) Root length (mm) Shoot length (mm)
 Trial 2 Control
140

120

100

80

60

40

20

0
1 2 3 4 5 6 7 8 9 10 11

Root length (mm) Shoot length (mm)

 Trial 2 A
Trial 2 B
120
70
100
60
80 50

60 40

40 30

20
20
10
0
1 2 3 4 5 6 7 8 9 10 11 0
1 2 3 4 5 6 7 8 9 10 11 12
Root length (mm) Shoot length (mm)
Root length (mm) Shoot length (mm)

Trial 2 C Trial 2 D
50 35
45
30
40
35 25
30 20
25
20 15
15 10
10
5
5
0 0
1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 11

Root length (mm) Shoot length (mm) Root length (mm) Shoot length (mm)
 Trial 3 Control
140

120

100

80

60

40

20

0
1 2 3 4 5 6 7 8 9 10 11

Root length (mm) Shoot length (mm)

 Trial 3 A Trial 3 B
140 90

120 80
70
100
60
80 50
60 40
30
40
20
20 10
0 0
1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 11

Root length (mm) Shoot length (mm) Root length (mm) Shoot length (mm)

Trial 3 D
Trial 3 C
70
90
60
80
70 50
60 40
50
30
40
30 20
20 10
10
0
0 1 2 3 4 5 6 7 8 9 10 11
1 2 3 4 5 6 7 8 9 10 11
Root length (mm) Shoot length (mm)
Root length (mm) Shoot length (mm)
 Average Control
140

120

100

80

60

40

20

0
1 2 3

Root length (mm) Shoot length (mm)

 Average A
Average B
90
80 70

70 60
60 50
50
40
40
30 30
20 20
10
10
0
1 2 3 0
1 2 3
Root length (mm) Shoot length (mm)
Root length (mm) Shoot length (mm)

Average C
50 Average D
45 25
40
35 20
30
25 15
20
15 10
10
5 5
0
1 2 3
0
Root length (mm) Shoot length (mm) 1 2 3

Root length (mm) Shoot length (mm)

 R/S Ratio Control
0.3

0.25

0.2

0.15

0.1

0.05

0
1 2 3
 R/S Ratio A
B
0.25
0.3

0.2 0.25

0.15 0.2

0.15
0.1
0.1
0.05
0.05

0
0
1 2 3
1 2 3

C D
0.3 16

14
0.25
12
0.2
10

0.15 8

6
0.1
4
0.05
2

0 0
1 2 3 1 2 3
 Germination Control
120%

100%

80%

60%

40%

20%

0%
1 2 3
 A B
120% 72%
70%
100%
68%
80% 66%
64%
60%
62%
40% 60%
58%
20%
56%
0% 54%
1 2 3 1 2 3

C
D
70%
45%
60%
40%
50% 35%

40% 30%
25%
30%
20%
20% 15%
10% 10%
5%
0%
1 2 3 0%
1 2 3