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Group 10 - Dumas Method
Group 10 - Dumas Method
Group 10
Abigail S.
JOURNAL
Elissa O.
Tiffany B.
REVIEW
OUTLINE
▣ About Journal
▣ Introduction
▣ Material and Methods
▣ Results and Discussion
▣ Conclusion
▣ References
▣ Recommendations
2
“Cereal protein analysis
via Dumas method:
Standardization of a
micro-method using the
EuroVector Elemental
Analyser”
3
About Journal
CITATION
4
INTRODUCTION
combusting a
sample of known
Dumas
mass at high
Method
temperature in the
presence of oxygen
To determine
the molecular
weight of an
unknown gas based on digestion
Kjeldahl
of the sample with
Method
sulphuric acid
5
DUMAS METHOD
(by Jean Baptiste Dumas in 1831)
7
Dumas Method gives a more reliable measure
of organic nitrogen than KM and offers greater
precision in the determination of protein
content in cereals and oilseeds
Advantages :
Faster
More economical
Less Polluting
Safer
8
The aim of this study was to
analyze the effect of certain ACFs on
the DM analytical process, using a
PlacketteBurman experimental
design to identify IFs and later to
optimize the IF combination.
MATERIAL & METHODS
▣ The Dumas method is based on combustion of
the whole sample in an oxygen-enriched
atmosphere at a high temperature in order to
ensure complete combustion.
▣ A EuroVector Elemental Analyser EA3000
equipped with Callidus software was used.
▣ Two samples obtained from the Community
Bureau of Reference (BCR).
▣ Certified reference materials (CRMs)
Analytical Instrument 10
MATERIAL & METHODS
▣ Two cereal reference materials :
-BCR-381 rye (Secale cereale L.) flour, 1.249 0.1 %N)
Reference Materials 11
MATERIAL & METHODS
Screening Phase 12
MATERIAL & METHODS
▣ Accuracy is the concordance between the detected
value obtained and the certified reference value, the
R1 (CRM-381) and R3 (CRM-382) responses will
measure the difference between both detected
(mean of four replicates) and certified values.
Optimization Phase 13
RESULTS AND DISCUSSION
15
▣ In order to validate the conditions suggested here for the use
of the DM for protein measurement in cereals, five
measurements were performed for Wheat (2.1067, 2.1253,
2.1254, 2.1365 and 2.1162, mean 2.122 e real values 2.12% and SD
0.011) and Rye (1.2534, 1.2728, 1.2655, 1.2685 and 1.2533, mean
1.262 cereal value 1.249%- and SD 0.009).
▣ Beljkas et al. (2010) concluded that since the DM procedure is
very simple and almost completely automated, there are
relatively few sources of uncertainty compared to the KM.
16
CONCLUSION
▣ Measurement of nitrogen content using the DM
requires the prior optimization of analytical
conditions for each sample type
▣ Application of a non-geometric PlacketteBurman
experimental design enabled the identification of
two critical factors:
-RFT (rear furnace temperature)
-GCT (GC oven temperatures)
17
RECOMMENDATION
+ -
▣ This journal ▣ There’s no
increases our conclusion part in
knowledge about this journal.
Dumas Method.
18
REFERENCES
AOAC, 1990. Official Methods of Analysis, 4th Supplement (1993). 992.23 Method, 15th ed.
AOAC International, Arlington, Virginia.
Beljkas, B., Matic, J., Milovanovic, I., Jovanov, P., Misan, A., Saric, L., 2010. Rapid method for
determination of protein content in cereals and oilseeds: validation, measurement
uncertainty and comparison with the Kjeldhal method. Accreditation and Quality
Assurance 15, 555e561.
Belliardo, J.J., Wagstaffe, P.J., 1988. BCR reference materials food and agricultural analysis:
and overview. Fresenius Zeitschrift für Analytische Chemie 332, 533e538.
Chiacchierini, E., Dáscenzo, F., Restuccia, D., Vinci, G., 2003. Milk soluble whey
proteins: fast and precise determination with Dumas method. Analytical Letters
36 (11), 2473e2484.
Daun, J.K., DeClercq, D.R., 1994. Comparison of combustion and Kjeldhal Methods
for determination of nitrogen in Oilseeds. Journal of the American Oil Chemists’
Society 71 (10), 1069e1072.
Kjeldahl, J., 1883. A new method for the determination of nitrogen in organic matter. Zeitschrift
fur Analytische Chemie 22, 366e382.
Thompson, M., Owen, L., Wilkinson, K.,Wood, R., Damant, A., 2002. A comparison of the
Kjeldahl and Dumas methods for the determination of protein in foods, using data
from a proficiency testing scheme. Analyst 127, 1666e1668.
Williams, P., Sobering, D., Antoniszyn, J., 1998. Protein testing methods at the Canadian grain
Commission. In: Wheat Protein Symposium, Saskatoon, Canada, March 9e10.
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