ISOLATION OF CAFFEINE

FROM A TEA
JOYCE ABIGAIL F. SORIANO
01 INTRODUCTION

02 PROCEDURES TABLE OF
CONTENTS
03 RESULTS

04 DISCUSSION
 01
INTRODUCTION
The tea, caffeine and isolation

TEA
-a beverage produced by
steeping in freshly boiled
water the young leaves and
leaf buds of the tea plant,
Camellia sinensis.
-Tea powder is extracted from
tea leaves which contain
tannins,
CAFFEINE
has a stimulating effect on the
central nervous system, heart,
blood vessels, and kidneys. It
INTRODUCTION
also acts as a mild diuretic.
It improves motor
performance, decreased
fatigue, enhanced sensory
activity, and increased
alertness.
 A method used for the
separation of organic
compound from a mixture of
EXTRACTION compound. This technique
selectively dissolves one or
more compounds into an
appropriate solvent.
 With this method, the
caffeine within a tea bag
could potentially be
EXTRACTION isolated to yield a pure
solid; the mass of this solid
would reflect the actual
yield of caffeine in the tea.
 Caffeine must be
introduced to a solvent
EXTRACTION that is both volatile and
insoluble to water; a
perfect example is
methylene chloride
02
PROCEDURES
 1. Open 3 commercial tea bags,
take the weights of the leaves.
Boil in 100 ml of distilled water for 5
mins. (Cover beaker with watch glass.)

SOLID-LIQUID
EXTRACTION 2. Extract liquid from the leaves.
Cool the aqueous tea extract to room
temperature in an ice bath.
 3. Extract the aqueous solution with 20
mol of dichloromethane (CH2Cl2) in a
separatory funnel.
after 2 mins transfer the
dichloromethane layer in a clean flask.
LIQUID-LIQUID
EXTRACTION 4. Repeat previous step twice with 20 ml
of fresh CH2Cl2.
Combine all CH2Cl2 extract. Discard the
aqueous tea layer.
 5. Transfer the combined CH2Cl2 extract
back to the separatory funnel and wash
this with 20 ml of 6 M NaOH solution.
Draw the CH2Cl2 layer onto a clean dry
flask containing half-a-spatula of
anhydrous Na2SO4. Decant onto an
EVAPORATION
evaporating dish.

6. Concentrate the CH2Cl2 layer over a

water bath and evaporate to dryness.

7. Calculate % yield.
 03
RESULTS
03
SOLID-LIQUID EXTRACTION

13
LIQUID-LIQUID EXTRACTION

14
EVAPORATION

15
 Weight of tea: 6.77 g
Weight of caffeine: 0.071 g

Calculations:

PERCENTAGE (0.071 g / 6.77 g) x 100

YIELD
=1.05 g
Theoretical Yield: 0.165 grams (3 tea
bags)

Actual Yield: 0.071 gram

%Error: 57%
04
DISCUSSION
What the role Sodium carbonate serves two main
of sodium functions:
carbonate • to place caffeine in a more basic
and sodium environment so that it has a
higher affinity for
sulfate in dichloromethane
the • to cause the tannins to form
isolation of phenolic salts in the aqueous
caffeine? solution.
Sodium sulfate was added to the extraction to absorb any water that escaped from the tea and the
remaining fluid was decanted and rinsed

What the role

of sodium
carbonate Sodium sulfate was added to the
and sodium extraction to absorb any water
that escaped from the tea. It is a
sulfate in drying agent
the
isolation of
caffeine?
Sodium sulfate was added to the extraction to absorb any water that escaped from the tea and the
remaining fluid was decanted and rinsed

What are • Emulsion is a mixture of two

emulsions? immiscible liquids
Why do • When tannins are converted to
their salts, they become an ionic
they form surfactant.
during • These causes the materials that
extractions don’t dissolve in water to form an
? How are emulsion with water.
they • This emulsion can be minimized
minimized? reducing the energy input through
slow and careful shaking and
swirling of the separatory funnel.
Sodium sulfate was added to the extraction to absorb any water that escaped from the tea and the
remaining fluid was decanted and rinsed

Why is it
necessary to
remove aa
stopper from a
It is necessary to remove the
separatory
stopper from a separatory
funnel when
funnel in order for the liquid to
liquid is being
continually flow out.
drained from it
through the
stopcock?
THANKS!
Sodium sulfate was added to the extraction to absorb any water that escaped from the tea and the
remaining fluid was decanted and rinsed

QUESTIONS: 1. A method used for the separation

of organic compound from a
mixture of compound
2. This compound was added to the
extraction to absorb any water
that escaped from the tea. It is a
drying agent
3-5. What are the three types of
extraction used for this
experiment.