“ANEXPERIMENTAL

“AN EXPERIMENTALEVALUATION
EVALUATIONON ON
AMLAPITTAHARAEFFECT
AMLAPITTAHARA EFFECTOF OF
PARIBHADRA(ErythrinaindicaLam.)AND
PARIBHADRA(Erythrinaindica Lam.)AND
YASHTIMADHU(Glychyrizaglabra.linn)
YASHTIMADHU(Glychyrizaglabra.linn)
- ACOMPARATIVE
-A COMPARATIVESTUDY.”
STUDY.”
BY
BY
DR.VIPIN
DR. VIPINKUMAR
KUMAR B.A.M.S
B.A.M.S
GUIDE:
GUIDE:
PROF.SANJAYA.
PROF. SANJAYA.K.K.S.S. B.Sc., M.D.(AY)
B.Sc., M.D.(AY)
PRINCIPAL
PRINCIPAL
DEPT.OF
DEPT. OFP.G.
P.G.STUDIES
STUDIESININDRAVYAGUNA
DRAVYAGUNA

DEPARTMENT OF POST GRADUATE STUDIES IN DRAVYAGUNA

DEPARTMENT OF POST GRADUATE STUDIES IN DRAVYAGUNA
A.L.N.RAO AYURVEDIC MEDICAL COLLEGE AND HOSPITAL
A.L.N.RAO AYURVEDIC MEDICAL COLLEGE AND HOSPITAL
KOPPA,CHIKAMAGALUR-577127
KOPPA,CHIKAMAGALUR-577127
INTRODUCTION
 Amlapitta is one among the commonest disorders
 Amlapitta is a disease of Annahava Srotas and is more common in
the present scenario of unhealthy diets
Dyspepsia is extremely prevalent, affecting up to 80% of the
population at some time, and very often no abnormality is
discovered during investigations especially in younger patients [<
40 years of age]. Hence, most of the patients fall under Non-Ulcers
dyspepsia / functional dyspepsia. Women are said to be affected
twice as commonly as men.

Tikta rasa dravyas can act effectively in the basic pathogenesis of

Amlapitta; Paribhadra being the Tikta rasa pradhana and Yestimadhu
a proven drug for Amlapitta but it is a Madhura Rasa pradhana rasa
dravyas so have been selected to evaluate their Amlapittahara
effect through experimental model
 OBJECTIVES
The study was based on the following objectives..

1. To experimentally evaluate the efficacy of Paribhadra

and Yashtimadhu in Amlapitta.
2. To evaluate and establish the most potent drug between
Paribhadara and Yashtimadhu
inAmlapitta.
3. Detailed pharmacognostic studies of Paribhadra and
Yashtimadhu
4. To do the phytochemical analysis of Paribhadra and
Yashtimadhu
DRUG REVIEW
PARIBHADRA (Erythrina variegata Linn.)
•It can be used in vamana along with shalmali, palasha and
kimshuka.
•It can be used with shalmayadi drugs in the preparation of
kalyanaka lavana which is useful over vata roga, gulma,
pleeha, agnimandya, ajeerna,arshas, arochaka, kasa and
krimi.
The Bhasma of paribhadra mixed with other drugs is useful over
purification of ashudha jala

•Panchangas of the plants are used according to the need

Rasa, Guna, Veerya, Vipaka

Rasa – Tikta, Katu.

Guna – Laghu.
Veerya – Ushna.
Vipaka – Katu
MORPHOLOGY OF PARIBHADRA
DRUG REVIEW-YASHTIMADHU
Yashtimadhu(Glycyrrhiza glabra Linn.) is a member of Fabaceae
family
•It is an important drug described in detail in Ayurvedic
classical texts. The references of this drug are also available
in the Samhitas and Nighantus

•It is having significant role in treating diseases like kasa,

shwasa, hikka, swarabheda, sukrameha

•Moola is the useful part.

Rasa-Madhura Guna- Guru,Snigdha Virya

-Seeta Vipaka- Madhura
MORPHOLOGY-YASHTIMADHU
DISEASE REVIEW
Acharya Charaka-
•While explaining about the qualities of milk it has been indicated
as pathya in Pandu roga , Amlapitta etc.
•While stating the effects caused due to excessive use of lavana
rasa, there is a
mention that it provokes pitta , lohita pitta, amlapitta etc .
•Amlapitta is also mentioned in the context of illeffects of vidhi
virudha ahara sevana.
•Vaghbhata has mentioned that excessive use of kulatha causes
Amlapitta .

•Kashyapa samhita first describes Amlapitta as a seperate

disease entity in khilasthana .and has explained its nidana,
lakshana , samprapti and chikitsa elaborately.
MATERIALS AND METHODS

•The present study is aimed to compare the Amlapittahara

effects of Paribhadra(Erythrinaindica Lam.) and Yestimadhu
(Glycyrrhizaglabra Linn.)based on the animal model Shay’s
method(Pyloric ligation)

•The present study has been carried out with four headings:
1. Collection and preservation of the drugs
2. Preparation of medicine
3. Pharmacognostical and phytochemical studies
4. Animal experimental study
 Grouping:
Sample 24 albino rats of either sex will be selected randomly divided into 4 groups.
Inclusion Healthy albino rats of either sex weighing in between 150-200g.
criteria
Exclusion Diseased rats, rats under trial for other experiments and rats below 150g and
criteria 200 g.

Group No.of Drug Form Dose Purpose

Rats

Control 6 Distilled water - 2ml/kg body wt. Toserveas

group prophylactic
control

Standard 6 Omeprazole Liquid 1.8mg/kg body Positive control

group (capsules) wt.

Trialgroup 6 Paribhadra Choorna 0.216gms/kg body To evaluate

1 (Erythrinaindica)leaves wt. Amla pitta hara
effect

Trialgroup 6 Yashtimadhu (Glycyrrhiza Choorna 0.216 gms/kg body To evaluate

2 glabra)Root wt. Amla pitta hara
effect
 Procedure & Criteria for assessment of the results:
Duration of the study- 8 Days
•The experiment will be carried out by following Shay’s method (pyloric ligation) for
the induction of hyperacidity and gastric ulcers 15.
•The control, standard and trial drugs will be administered to albino rats of respective
groups in appropriate dosage with normal food and water for 5days.
•On 6th and 7th day rats will be kept on fasting and only the drugs will be administered.
•On the 7th day, 1 hr after giving the drug, rats are to be anaesthetized, abdomen is
opened and pyloric ligation is done.
•Abdomen is to be closed with sutures.
•16 hrs after pyloric ligation, rats are to be sacrificed and gastric juice which is
collected from the stomach is to be sent for analysis. The stomach is cut open along
greater curvature and examined for ulceration .
After 16 hrs of pyloric ligation, rats are to be sacrificed and stomach was removed
for the observation of-
•Ulcer score
•Ulcer index
•Free acidity
•Total acidity and
•pH
. Showing Florescent Tests of Leaves of Erythrina indica Lam.
Color Under Visible Light Color Under Long UV
Sample Mud green Green

Sample + Water Yellowish pale green Dark green

Sample + Methanol Green Fluorescent orange

Sample + 10% NaOH Pale green Yellowish brown

Sample + 10% HCl Pale yellow Pale yellow

Sample + 10% HNO3 Pale greenish brown Brown

Sample + 10% H2SO4 Pale greenish brown Brown

Sample + 10% NH3 Yellowish green Pale green

Showing Florescent Tests of Root of Glycyrrhiza glabra Linn.
Color Under Visible Light Color Under Long UV

Sample Creamish pale yellow Bright fluorescent yellow

Sample + Water Creamish yellow Brown

Sample + Methanol Pale yellow Creamish green

Sample + 10% NaOH Olive green Yellowish brown

Sample + 10% HCl Pale yellow Yellowish green

Sample + 10% HNO3 Brownish yellow Yellowish green

Sample + 10% H2SO4 Brownish yellow Fluorescent pale green

Sample + 10% NH3 Bright yellow Yellowish brown

Results of Thin Layer Chromatography
TLC-1. Leaves of Erythrina indica Lam

Before Spray
Under Long UV Under Visible Light
Rf
0.09 Pale blue ---
0.37 Pale fluorescent blue ---
0.52 Pale fluorescent blue ---
0.81 --- Bluish gray

After Spray
Under Long UV Under Visible Light
Rf
0.09 Pale blue ---
0.37 Pale fluorescent blue Pale yellow
0.52 Pale fluorescent blue ---
0.81 Red Creamish orange
TLC- II : Root of Glycyrrhiza glabra Linn.
Before Spray
Under Long UV Under Visible Light
Rf
0.05 Pale blue ---
0.22 Pale blue ---
0.30 Pale blue ---
0.37 Fluorescent blue ---
0.52 Pale blue ---

After Spray
Under Long UV Under Visible Light
Rf
0.09 Fluorescent blue Pale creamish brown
0.37 Fluorescent blue ---
0.52 Fluorescent blue Pale yellow
0.81 Purple ---
Showing Ulcer index in treated rats
20

18.08

15

9.99
10 9.58

5
4.06

0
control standard trail drug 1 trail drug 2
Showing % of inhibition of ulcer in treated rats
100.00%

81.13%

50.55%
50.00% 48.13%

0.00%
standard trail drug 1 trail drug 2
Showing volume of gastric juice in treated rats
15

13.15

10
9.33

8.3

5.19
5

0
Control Standard Trail Drug 1 Trail Drug 2
Showing pH in treated rats
6

4 3.87

3.49 3.52

2.15
2

0
Control Standard Trail Drug 1 Trail Drug 2
Showing free acidity of all groups
6
5.68

4.1
4 3.86

3.2

0
Control Standard Trail Drug 1 Trail Drug 2
 Showing total acidity of all groups
15

10.84

10

7.4
7.1

5.6

0
Control Standard Trail Drug 1 Trail Drug 2
DISCUSSION
Drug Review‒

Yashtimadhu
The drugs which are taken up for this study are namely Paribhadra
and Yashtimadhu.

Yashtimadhu is familiar since Purana Kala to the present era. It is

used in various ailments as quoted by various lexicographic texts and
various synonyms.
Vernacular names of the plant Yashtimadhu portray its image as an
entity beneficial for conception. The stolons as well as root of the plant
are used in a variety of ailments. For the present study the root of
Yashtimadhu was selected which was botanically identified as
Glycyrrhizaglabra Linn.
Rasapanchaka of Yashtimadhu is as follows –
Rasa :Madhura, Tikta, Guna : Guru, Snigdha,;
Virya: Sheeta; Vipaka: Madhura
 Paribhadra

Paribhadra is more familiar from the Samhita Kala to present era, not
mentioned in Purana or veda kala. It is also mentioned in various
diseases as quotedlexicographic texts and various synonyms.
For the present study the leaf of Paribhadra was selected which was
botanically identified as Erythrinavariegata Linn.
Rasapanchaka of Paribhadra is as follows –
Rasa :Tikta,Katu
Guna :Laghu;
Virya: Ushna;
Vipaka: Katu.
Discussion on Disease
Amlapitta is a psycho-somatic disease of annavaha srotas mainly caused due to
indulgence in faulty diet and regimen. It presents a group of signs and
symptoms viz., avipaka, amlodgara, hrtdaha, kantadahautklesha, vamana,
udaarashoola, adhmanavitbheda etc. which are more commonly termed as
dyspeptic signs in the modern medicine.

Amlapitta is most commonest disease met with in general practice requiring

an adequate and proper management as it hampers the daily routine of an
individual.
Madhavakara states that Amlapitta is a condition which and when promptly
treated along with proper diet and regimen can be easily cured. If it is neglected
and one indulgence himself in apathyaaharavihara it becomes kastasadhya
CONCLUSION
The present study entitled“An experimental evaluation on Amlapittahara
effect of Paribhadra Patra (Erythrinaindica Lam.) and Yashtimadhu
(Glycyrrhizaglabra Linn.)-A comparative study”was under taken to assess the
efficacy of the two trial drugs as Amlapittahara.
The following conclusions can be drawn on the action of the trial drugs based
on the result of experiment on Amlapitta:
The present experimental study was undertaken by adopting “Pyloric ligation”
method introduced by Shay et al.,1945on Albino rats.
The out come of the result showed both the Trial drugs having significan
tAmlapittahara effect when compared to control group.
On comparing the trial drugs,both Paribhadra Patra (Erythrinaindica Lam.) and
Yashtimadh(GlycyrrhizaglabraLinn.) exhibited similar action statistically, but the
action of Yashtimadhu (Glycyrrhizaglabra Linn.) was found to be better than
Paribhadra Patra (Erythrinaindica Lam.) when mean values were compared.
When compared with the standard drug Omeprazole, Paribhadra
Patra(Erythrinaindica Lam.) was less effective and Yashtimadhu
(Glycyrrhizaglabra Linn.) was equally effective to Omeprazole