Fungi /Jamur

Prof. Dr. Mochammad Hatta, PhD, SpMK (K)

Bagian Mikrobiologi
FK, UNHAS
 Kingdom Fungi

• Terdiri dari >100,000 species dibagi

menjadi 2 groups:
– macroscopic fungi ( mushrooms/jamur)
– microscopic fungi (molds, yeasts/ragi)
• Dari >1oo,000 species yang ditemukan 
± 100 spesies patogen bagi manusia.

4
Morfologi
yeast

5
 Hyphae / Molds
• Multicellular, filamentous fungi (Long filaments of cells
joined together)
• Identified by physical appearance, colony characteristics, and
reproductive spores.
• Hiphae:
– Septate hyphae: Cells are divided by cross-walls (septa).
– Coenocytic (Aseptate) hyphae: Long, continuous cells that are not
divided by septa.
• Part of hiphae:
– Vegetative Hypha: Portion that obtains nutrients.
– Reproductive or Aerial Hypha: Portion connected with reproduction.
• Mycelium: Large, visible, filamentous mass made up of many
hyphae.
Morfologi hiphae/molds

7
Morfologi Hiphae / Molds
Mycelium: Large, Visible Mass of Hyphae
 Dimorphic Fungi
Can exist as both multicellular fungi (molds) and
yeasts.
Many pathogenic species.
 Mold form produces aerial and vegetative hyphae.
 Yeast form reproduces by budding.

Dimorphism in pathogenic fungi typically depends on

temperature:
 At 37oC: Yeast form.
 At 25oC: Mold form.

Dimorphism in nonpathogenic fungi may depend on

other factors: Carbon dioxide concentration
 Mycoses
 Merupakan infeksi kronis, karena
pertumbuhan jamur yang lambat
 Klasifikasi:
1. Superficial mycoses
2. Cutaneus mycoses
3. Subcutaneus mycoses
4. Systemic mycoses
5. Opportunistic mycoses
 General Clinical Classification of Pathogenic
Fungi

Superficial Cutaneus Subcutaneus Sistemic Opportonistic

•Pityriasis •Tinea pedis •Chromoblasto •Aspergillosis •Aspergillosis
versicolor •Tinea mycosisi •Blastomycosis •Candidosis
•Tinea niegra unguinum •Sporothricosis •Candidosis •Cryptococcosis
•Piedra •Tinea corporis •Mycetoma •Coccidioidomyc •Zygomycosis
•Tinea cruris •Phaeohypomy osis
•Tinea manus cois •Histoplasmosis •Geothricosis
•Tinea capitis •Cryptococcosis •Fusariosis
•Tinea barbae •Trichosporonosis
•Geothrichosis •Others
•Paracoccidioido
mycosis
•Zygomycosis
•Fusariosis
•Trichosporonsis
 Diagnosis laboratorium
• Spesimen: skin scrapping (kerokan kulit),
potongan rambut  pengecatan dengan
KOH 10-20%  diamati dibawah mikroskop
Laboratoy Diagnosis Dermatophytosis:
• Spesimen: Skin Scrapings, nail scrapings and
epilated hairs

• Method:
– Direct Microscopy: KOH 10-20% and ink parker
– Culture: Sabouraud's dextrose agar containing
cycloheximide incubated at 26-28C for 4 weeks
 Microscopic & Culture

Skin scrapping: Endothrix: spore

Exothrix: spore
hiphae

Culture on SDA
 Laboratory diagnosis candida spp
Clinical specimen:
• Skin scrapings,
• Mucosal scrapping,
• Vaginal secretions
• Blood ans other body fluid

Method:
• Direct mikroskopic
• Cultures : on Sabouraud's Glucose agar
• PCR
 Laboratory Diagnosis Criptococcus.

• Mikroskopic: Indian ink staining,

Gram staining
• Kultur :Cultures on Sabouraud
dextrose agar
• Serologis: detection of Capsular
antigen
 Coccidioides immitis

Disseminated
coccidiomycosis

mochammad hatta@2013
 Blastomyces dermatitidis

mochammadCutaneous
hatta@2013 blastomycosis
 lymphocutaneous sprotrichosis

Primary sore and series of secondary nodules along lymphatic chain in the arm

mochammad hatta@2013
 Mycetoma

mochammad hatta@2013
Ringworm Tinea capitis – T.corporis
mochammad hatta@2013
 Ringworm / Dermatophytosis/T.cruris, T.manuum,. T.pedis

mochammad hatta@2013
 Tinea
versicolor

mochammad hatta@2013
 Candida albicans

Thrush

Candidal diaper
rash

Vaginitis

mochammad hatta@2013
 Cryptococcus neoformans

mochammad hatta@2013
 Aspergillus

Conjunctival infection Brain abscesses (darkened areas)

mochammad hatta@2013
Terima kasih