Analytical Test variables

presented by Hajrah Farooq

Aatika Maryam
Learning Objectives
1. Describe Test Variable Knowledge.
2. Describe different types of Test variables Knowledge.
3. Discuss the steps taken to prevent pre-analytical and post-analytical errors.
4. Discuss the steps taken in the lab to prevent analytical errors.
Stages:

 The diagnostic testing process can be separated into three phases:

 (a) The Pre-analytical stage
 It consists of patient preparation and sample collection. E.g - Blood or Urine &
sending it to Lab
 (b) The Analytical stage
 The analytical phase consists of processes involved in the testing of the specimen
 (c) The Post-analytical stage
 It refers to the interpretation of test to formulate a diagnosis.
Types of Variables

There are some variables that can dramatically affect the test results and are
classified accordingly
1. Pre analytical variables (62%)

2. Analytical variables (15%)

3. Post analytical variables (23%)

Pre-Analytical Variables
Pre-analytical errors

 By clinicians:  By nursing staff

 Hand writing not legible  Form lost or delayed

 Request written on wrong form  Improper preparation of patient
 Patient identification incorrect  Wrong position of patient at time
of sample collection
 Errors before, during and after collection of
sample

Before collection During collection After collection

 Patient  Extended • Specimen lost or
misidentification tourniquet delayed
• Unsuitable storage
 Improper time of  Haemolysis
and conditions
collection
 Under filling tubes • Failure to
 Exercise separate serum
 Blood diluted with
 Improper posture IV fluid from sample
• Improper light
 Fasting exposure
• Wrong tube /
contamination
from stopper
Analytical phase variables
Analytical protocols

 Skill of the technician.

 Fresh reagent must be used and not the expired one.
 Instrument maintenance must be done as it must be checked periodically
by the staff.
 Equipment used must be properly calibrated.
 Test procedure must be appropriate with proper mixing of the components.
 Dilution and pipetting errors must be reduced.
Post analytical variables
 Post analytical protocols

 1. Proper entry of lab results

 Entry on the form
 Entry on record register

 2. Pathologist signature & interpretation

 Results should be shown to pathologist for interpretation & rectification

 3. Immediate delivery of results for cases requiring urgent consideration

 4. Attachment of reports on case sheet
 Immediately
 With the case sheet of actual patient

 5. Clinician knowledge/correlation of results

Post analytical errors

 Incorrectly transcribed data.

 Not reported in timely manner.
 Reported in a confusing or uninterpretable format.
 Copying mistake from a raw data sheets onto patient record chart.
 Acronyms are often used to shorten the test names listed on reports or
computer monitor screens.
EXAMPLE:
The acronym "LAP" can stand for "leucine amino peptidase" and also for
"leukocyte alkaline phosphatase."
The computer code "ALDO" could be applied to both aldolase and
aldosterone.
EXAMPLE:
 If the reference range for albumin is 3.5 to 5.0 g/dL and an unrealistic
patient value of 0.35 g/dL is identified, this may alert the analyst to an
instrumental, mathematical, or decimal positioning error, which may have
caused a 10-fold error from an expected value of 3.5 g/dL
Steps to prevent pre and post
analytical errors
 Phlebotomy education:
All employees should be required to take continuing education classes to stay
current with recent developments in pre-analytical error reduction.
 Using appropriate technology:
Barcodes for correct patient identification.
Automated detection of serum indices.
 Choosing appropriate products/instruments:
Plastic tubes offer significant advantages over glass tubes, such as minimizing
exposure to blood by reducing the chance of shatter, increasing shock
resistance, increasing centrifugation speed tolerance, and decreasing weight.
 Developing clear, written procedures:
Specific protocols and operating procedures (SOPs) in the lab help to reduce
the heterogeneity in process and streamline the lab workflow.
 Validating any new instrument or procedure.
 Monitoring quality indicators in the lab:
Laboratory General Checklist of the College of American Pathologists (CAP)
specifically lists a few such pre-analytical quality indicators which should be
monitored:
a. Patient identification (% of patient wristbands with errors, % of ordered tests
with patient identification errors, % of results with identification errors)
b. Test order accuracy (% of test orders correctly entered into a laboratory
computer)
c. Specimen acceptability (% of general Hematology and/or Chemistry
specimens accepted for testing).