General Microbiology Laboratory

Introduction to Food Microbiology

Laboratory
 Laboratory Safety Rules

1. Wear a lab coat in lab. We will be working

with a variety of materials that can cause
permanent stains on some fabrics. Also, a lab
coat can help protect from accidental
contamination by microorganisms

No eating or drinking during lab. Many

pathogens spread by ingested food and drink.
In addition, food can carry microorganisms
that might contaminate laboratory cultures
 Laboratory Safety Rules

Keep long or fluffy hair tied up and out of the

way. Hair can contaminate and be
contaminated by microbial cultures.

Always wear shoes in lab

Thoroughly wash your hands with soap and

water before and after lab. Thorough and
frequent hand washing easily and effectively
controls the spread of many pathogens.
 Laboratory Safety Rules

Clean the lab bench with disinfectant

before and after lab. This helps to
prevent contamination of cultures,
books, clothing, etc

Keep the lab bench free of

unnecessary materials. Don't use the
lab bench as a storage area for coats,
books, etc.

Do not take cultures from the lab area

 Laboratory Safety Rules
 Dispose all contaminated materials in autoclave bags.
When in doubt, ask the instructor.

 Immediately report all accidents and spills to the

instructor. Cover spills with disinfectant-soaked paper
towels for at least 15 minutes before disposing them

 Read all assigned materials before the lab session.

Experiments will go smoother and have greater chances
of success when you know what you will be doing ahead
of time
 Laboratory Safety Rules

Treat all microbial cultures as if they

are pathogens. Better safe than sorry

When in doubt, ask the instructor.

The only stupid questions are those that

are intended as such.
Personal belongings are not to be stored
in the laboratory.
 Lab requirements

Lab coat
Matches or lighter
Soap
Waterproof permanent marker
Small or medium size towel
Sealable plastic bag
Rubber band
 Compiled laboratory activities
Acquaint yourself with microbiology lab

Microscope

Alcohol lamp
Lysol

Microscopic slides
Staining solutions
Acquaint yourself with microbiology lab

Forceps
Sterile swabs

Bunsen burner

Wooden cotton swabs

Acquaint yourself with microbiology lab

Incubator

Autoclave
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Laminar flow Hood

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Culture Plates or Petri

Dishes

Flaming Nichrome
loop
Wire Loops

Plastic Loops and Needles

Acquaint yourself with microbiology lab

Colony Counter

Anaerobic Jar
Acquaint yourself with microbiology lab

Anaerobic station
Acquaint yourself with microbiology lab

The Enterotube is an example of

a rapid, multi test system used
in identification of unknown
oxidase- negative, gram-
negative, rod shaped bacteria of
the family Enterobacteriaceae. It
consists of a tube with a flat side
and contains 12 compartments for
different biochemical tests.

Enterotube

API 20 E
Acquaint yourself with microbiology lab

Liquid media in tubes Solid media in tubes

Acquaint yourself with microbiology lab

Different forms of slants

Acquaint yourself with microbiology lab

Blood culture bottles

Acquaint yourself with microbiology lab

Antibiotic disks Pipettes

 Now

Try to Identify all the tools and

Equipment around you