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EXPERIMENT Enzyme Concentration
EXPERIMENT Enzyme Concentration
EXPERIMENT Enzyme Concentration
0.8
0.6
0.4
0.2
0
0.17 0.33 0.5 0.67 0.83 1
Concentration of amylase (%)
Discussion:
The time taken for amylase to
hydrolyse starch decreases as the
enzyme concentration increases.
A higher concentration of the
enzyme contains more enzyme
molecules to hydrolyse the starch
molecules. Hence, it takes less
time for the hydrolysis of starch to
be completed.
From the graph, it can be seen that
the rate of reaction increases when
enzyme concentration increases
until a certain concentration, when
the further increase in enzyme
concentration will not increase the
rate of reaction. This is because
the substrate concentration has
become the limiting factor.
Conclusion:
The rate of reaction increases with
the increase in enzyme
concentration until a certain
concentration of enzyme is
achieved.