Addition of 40 mM NaCl decreases frond counts and increases number of yellow fronds

compared to the control group for all Lemna minor clones studied, after 14 days of experiment.
A. LM.00027 B. LM.00132

Control Day 14 Experimental Day 14 Control Day 14 Experimental Day 14

C. LM.00142 D. LM.00191

Control Day 14 Experimental Day 14 Control Day 14 Experimental Day 14

Figure 1. 40mM NaCl was added to the Appenroth Media for the following Lemna minor clones:
A. LM.00027, B. LM.00132, C. LM.00142, D. LM.00191. The images displayed were taken after 14
days. N=2 samples were analyzed.
The growth of fronds after 14 days is diminished by the addition of 40mM
NaCl as compared to the control condition. N=2 samples were analyzed.
Average Number of Fronds

LM.00027 Number of Fronds vs. LM.00132 Number of Fronds vs.

Average number of fronds

Days Days
400 200
R² = 1 R² = 0.96 Total Frond Counts at 14 Days for Different
0
0 R² =2 1 4 6 8 10 12 14 16 100 Lemna minor clones
R² = 1

Total Number of Fronds

Time elapsed (days) 0 300 264.5
0 2 4 6 8 10 12 14 16
250 212
Time elapsed (days) 200 160
179
Control 143
150 112 108.5
Exponential (Control)
100 61
NaCl Control
50
Exponential (NaCl) Exponential (Control)
0
Exponential (NaCl) Pollutant LM.00027 LM.00132 LM.00142 LM.00191
Exponential (NaCl) Exponential (Pollutant)
Lemna minor Clone

LM.00142 Number of Fronds vs. LM.00191 Number of Fronds vs.

Days Days Control Experimental
Average Number of Fronds

Average Number of Fronds

250 200 Figure 2. This bar graph summarizes the

200
150
R² = 1 150 final counts (after 14 days) of the data
R² = 1 100 R² = 1
100 R² = 0.99 displayed in the graphs on the left. For
50 50
0 0 LM.00027, LM.00132, and LM.00191, the
0 2 4 6 8 10 12 14 16 0 2 4 6 8 10 12 14 16
control group displayed more frond
Time elapsed (days) Time elapsed (days)
counts. For LM.00142, the control group
displayed fewer frond counts.
Control Ctrl
Exponential (Control) Exponential (Ctrl)
NaCl NaCl
Exponential (NaCl) Exponential (NaCl)

Figure 1. The graphs for LM.00027, LM.00132, and LM.00191 indicate

that Lemna minor clones experience less growth when 40mM NaCl is
added. LM.00142 demonstrates the opposite conclusion. The
exponential trendlines show very strong correlations (all r2 > 0.950).