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Alloimmunisation To Blood Group Antigens
Alloimmunisation To Blood Group Antigens
Antigens
“Diagnostic Approach”
Primary IR Secondary IR
◦ Slow (weeks to ◦ Rapid
months ◦ Ab titer rising 48 hrs
◦ Low level of IgM ◦ IgG , peaks 6 days
◦ Needed high dose of after injection
Ag. ◦ Need smaller Ag dose
Immune Response to Blood Group Antigens
◦ Blood transfusion
◦ pregnancy
Immune Response to Blood Group Antigens
Immunogenicity
◦ The ability of an antigen to stimulate an immune
response or antibody production.
◦ Most blood group antigens are poor immunogens
(<1% of transfused patients).
Liquid-phase (tube/plate)
4+ 4+ 3+ 2+ 1+ 0
Column-agglutination (gel cards) method
Gel Card Method
Gel Card Method
Gel Card Method
Gel Card Method
Gel Card Method
Gel Card Method
Gel Card Method
Gel Card Method
Gel Card Method
Problems with current methods
0.14
0.12
Mean OD (minus
0.1
blank)
0.08 anti-K
0.06 anti-Kpa
0.04 anti-k
0.02
0
srFLAG-K/Kpa/Jsa K300
FLAG-Kell fusion protein
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