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  • Determinación de Pi: Aa Asp Gly Pro Phe Tyr His Glu Carga Neta Grupos Pka

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    Julián Idárraga
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    The document contains a table listing amino acids and their pKa values. It then performs a calculation to determine the net charge of each amino acid at different pH values. The final calculation determines that there is no true isoelectric point at pH=0, but pH=4.055 is the value that comes closest to having no net charge.

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    Explicación de pI

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    The document contains a table listing amino acids and their pKa values. It then performs a calculation to determine the net charge of each amino acid at different pH values. The final calculation determines that there is no true isoelectric point at pH=0, but pH=4.055 is the value that comes closest to having no net charge.

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    © All Rights Reserved
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    6 views6 pages

    Determinación de Pi: Aa Asp Gly Pro Phe Tyr His Glu Carga Neta Grupos Pka

    Uploaded by

    Julián Idárraga
    The document contains a table listing amino acids and their pKa values. It then performs a calculation to determine the net charge of each amino acid at different pH values. The final calculation determines that there is no true isoelectric point at pH=0, but pH=4.055 is the value that comes closest to having no net charge.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    Determinación de pI

    AA Asp Gly Pro Phe Tyr His Glu  


    GRUPOS NH2 R=COOH ----- ------ ------- OH NH2 R=COOH -COOH CARGA
    NETA
    pKa
    Determinación de pI
    AA Asp Gly Pro Phe Tyr His Glu  
    GRUPOS NH2 R=COOH ----- ------ ------- OH NH2 R=COOH -COOH CARGA
    NETA
    pKa 9,82 3,86 0 0 0 10,07 6,00 4,25 2,19

    0 – 2,19
    2,19 - 3,86
    3,86 – 4,25
    4,25 – 6,00
    6,00 – 9,82
    9,82 – 10,07
    10,07 – 14,00
    Determinación de pI
    AA Asp Gly Pro Phe Tyr His Glu  
    GRUPOS NH2 R=COOH ----- ------ ------- OH NH2 R=COOH -COOH CARGA
    NETA
    pKa 9,82 3,86 0 0 0 10,07 6,00 4,25 2,19

    0 – 2,19 +1 0 0 0 0 0 +1 0 0 +2
    2,19 - 3,86 +1 0 0 0 0 0 +1 0 -1 +1
    3,86 – 4,25 +1 -1 0 0 0 0 +1 0 -1 0
    4,25 – 6,00 +1 -1 0 0 0 0 +1 -1 -1 -1
    6,00 – 9,82 +1 -1 0 0 0 0 0 -1 -1 -2
    9,82 – 10,07 0 -1 0 0 0 0 0 -1 -1 -3
    10,07 – 14,00 0 -1 0 0 0 -1 0 -1 -1 -4

    =
      4,055
    Determinación de pI
    AA Glu Asp Arg His Glu Leu Phe Tyr Tyr Trp Glu  
    GRUPOS NH2 R=COOH COOH NH2 NH2 COOH ----- ----- OH OH ----- R=COOH -COOH CARGA
    NETA
    pKa
    Determinación de pI
    AA Glu Asp Arg His Glu Leu Phe Tyr Tyr Trp Glu  
    GRUPOS NH2 R=COOH COOH NH2 NH2 COOH ----- ----- OH OH ----- R=COOH -COOH CARGA
    NETA
    pKa 9,67 4,25 3,86 12,48 6,00 4,25 0 0 10,07 10,07 0 4,25 2,19
    Determinación de pI
    AA Glu Asp Arg His Glu Leu Phe Tyr Tyr Trp Glu  
    GRUPOS NH2 R=COOH COOH NH2 NH2 COOH ----- ----- OH OH ----- R=COOH -COOH CARGA
    NETA
    pKa 9,67 4,25 3,86 12,48 6,00 4,25 0 0 10,07 10,07 0 4,25 2,19

    0 – 2,19 +1 0 0 +1 +1 0 0 0 0 0 0 0 0 +3
    2,19 – 3,86 +1 0 0 +1 +1 0 0 0 0 0 0 0 -1 +2
    3,86 – 4,25 +1 0 -1 +1 +1 0 0 0 0 0 0 0 -1 +1
    4,25 – 6,00 +1 -1 -1 +1 +1 -1 0 0 0 0 0 -1 -1 -2
    6,00 – 9,67 +1 -1 -1 +1 0 -1 0 0 0 0 0 -1 -1 -3
    9,67 – 10,07 0 -1 -1 +1 0 -1 0 0 0 0 0 -1 -1 -4
    10,07 – 12,48 0 -1 -1 +1 0 -1 0 0 -1 -1 0 -1 -1 -6
    12,48 – 14,00 0 -1 -1 0 0 -1 0 0 -1 -1 0 -1 -1 -7

    𝑁𝑜
      h𝑎𝑦 𝑝𝑢𝑛𝑡𝑜 𝑖𝑠𝑜𝑒𝑙 é 𝑐𝑡𝑟𝑖𝑐𝑜 𝑐𝑜𝑛 𝑢𝑛 𝑣𝑎𝑙𝑜𝑟 𝑑𝑒 0 , 𝑝𝑒𝑟𝑜h𝑎𝑦 𝑢𝑛 𝑝𝐻 𝑞𝑢𝑒 𝑠𝑒 𝑎𝑐𝑒𝑟𝑐𝑎

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