Genome Assembly

Data
Aedes aegypti
 AedesF5AOL
# contigs (>= 0 bp) 314759 Total length 999516386
# contigs (>= 1000 bp) 139718 Reference Length 1278732104
# contigs (>= 5000 bp) 31593 GC % 38.33
# contigs (>= 10000 bp) 16126 Reference GC % 38.13
# contigs (>= 25000 bp) 7591 N50 21596
# contigs (>= 50000 bp) 3533 NG50 8056
Total Length (>= 0 bp) 1027536030 N75 4086
Total Length (>= 1000 bp) 949605930 NG75 904
Total Length (>= 5000 bp) 714752225 L50 8619
Total Length (>= 10000 bp) 608711196 LG50 19553
Total Length (>= 25000 bp) 475885130 L75 39316
Total Length (>= 50000 bp) 332527005 LG75 149652
# contig 209299
Largest contig 627755
 AedesF5AOL
# misassemblies 5209 Largest alignment 627755
# misassembled contigs 4444 Total aligned length 970548911
Misassembled contigs length 4715966 NA50 21536
# local miassemblies 20675 NGA50 7977
# scaffold gap ext. mis. 0 NA75 3919
# scaffold gap loc. mis. 0 NGA75 525
# unaligned mis. contigs 6125 LA50 8631
# unaligned contigs 3864 + 9967 part LGA50 19627
Unaligned length 13140529 LA75 39869
Genome fraction (%) 73.855 LGA75 179362
Duplication ratio 1.044
# N’s per 100 kbp 0.00
# mismatches per 100 kbp 1108.37
# indels per 100 kbp 107.86
 BUSCO – Aedes IDBA
Complete BUSCOs (C) 81.6%

Complete and single-copy BUSCOs (S) 67.9%

Complete and duplicated BUSCOs (D) 13.7%
Fragmented BUSCOs (F) 14.6%
Missing BUSCOs (M) 3.8%
Total BUSCO groups searched 1066
 BUSCO – Unmapped
Complete BUSCOs (C) 4.6%

Complete and single-copy BUSCOs (S) 4.3%

Complete and duplicated BUSCOs (D) 0.3%
Fragmented BUSCOs (F) 11.8%
Missing BUSCOs (M) 83.6%
Total BUSCO groups searched 1066
 BUSCO – AedesF5AOL
Complete BUSCOs (C) 75.8%

Complete and single-copy BUSCOs (S) 74.4%

Complete and duplicated BUSCOs (D) 1.4%
Fragmented BUSCOs (F) 8.3%
Missing BUSCOs (M) 15.9%
Total BUSCO groups searched 1066
Abyss Contigs
Abyss 91 Abyss 191 Abyss 195
# contigs (>= 0 bp) 4337854 1113165 1083707
# contigs (>= 50000 bp) 0 127 151
Total Length (>= 0 bp) 1822163718 1948979045 1950933315
Total Length (>= 50000 bp) 0 7539633 8929572
# contig 590303 461424 463898
Largest contig 40539 130604 130612
Total length 1130232559 1738733350 1748714940
GC % 37.66 37.85 37.85
N50 3097 8148 8271
N75 1628 4040 4047
L50 108366 61940 61095
L75 232627 136990 135982
# N’s per 100 kbp 51.93 6.10 7.12
Abyss Unitigs
Abyss 91 Abyss 191 Abyss 195
# contigs (>= 0 bp) 5261868 1625386 1581520
# contigs (>= 50000 bp) 0 11 11
Total Length (>= 0 bp) 1854943039 1902457203 1902232075
Total Length (>= 50000 bp) 0 719948 724660
# contig 763898 672475 674158
Largest contig 29726 100616 100624
Total length 1051793042 1614847704 1624747972
GC % 37.58 37.79 37.80
N50 1708 4259 4302
N75 965 1963 1967
L50 174888 105819 104731
L75 381682 244585 243725
# N’s per 100 kbp 0.00 0.00 0.00
 Abyss Scaffolds
Abyss 91 Abyss 191 Abyss 195
# contigs (>= 0 bp) 4273315 1062653 1034116
# contigs (>= 50000 bp) 2 651 714
Total Length (>= 0 bp) 1820103832 1945358772 1947263568
Total Length (>= 50000 bp) 145690 40988778 45105732
# contig 542135 417453 420797
Largest contig 75205 154423 154467
Total length 1130294804 1736728224 1746668755
GC % 37.66 37.84 37.85
N50 3896 10253 10389
N75 1768 4900 4884
L50 80421 47268 46766
L75 187005 108130 107567
# N’s per 100 kbp 177.86 72.55 72.58