Transport

Across the
Membrane
 Cellophane as an
Alternative
Semipermeable
Membrane in the
Mechanism of Dialysis

2
Hypothesis
• According to Cruz et. al (2019) dialysis refers
to the separation of molecules on the basis of
size and by their ability to diffuse through a
semipermeable membrane.

• A semipermeable membrane is a thin layer of

filtering material which separates substances
when a driving force is applied across the
membrane..

3
Hypothesis
• Cellophane is an accidental discovery by Swiss
chemist Jacques E. Brandenberger in 1900.
Inspired by seeing a wine spill on a restaurant’s
tablecloth, he decided to create a cloth that could
repel liquids rather than absorb them. He tried a
waterproof coating formulated from cellulose. The
coated fabric was too stiff, but the clear film
easily separated from the fabric; the film later
became known as cellophane.

4
The experimenters believe that
cellophane is not an effective
alternative semipermeable
membrane in the process of
dialysis.

5
 Experimentation

Materials
◍ 1 sq.foot cellophane
◍ Filter paper
◍ Evaporated milk
◍ String
◍ 500 mL beaker
◍ Distilled water
◍ 25% trichloroacetic solution
◍ Test tube

6
7
Dialysis

8
 Data Presentation

Test for protein Test for sugar

The solution is still clear, no white No red or yellow precipitate is

precipitate and no turbidity is present
present

9
 Result

Test for Protein Test for Sugar

Movement outside The movement outside the dialysis did
the Dialysis bag not occur maybe it is because of the
color of the cellophane used in the
did not occur,
experiment that affects the results.
the protein is a
larger molecule Expected result:
as it therefore The movement outside the dialysis only
it prevents to occurred in glucose because it is a
smaller molecule,therefore there is an
move across the
ability to pass through the membrane
membrane into the beaker
10
 Conclusion

Only smaller molecules can pass through the

membrane. From the result of this experiment, we have
failed to conduct the right procedure because of some
possible errors that occurred during the actual
experiment. The material used is one of the main error of
the experiment, The cellophane, not all cellophane is
permeable which doesn’t allow all kinds of substances to
pass readily through the cellophane that caused the
distilled water to pass through successfully.

11
 Credits

RESOURCES
https://www.sciencedirect.com/topics/engin
eering/semipermeable-membrane

https://chem.libretexts.org/Bookshelve
s/Physical
and Theoretical Chemistry Textbook
Maps/Supplemental Modules (Physical
and Theoretical Chemistry)/Physical
Properties of Matter/Solutions and
Mixtures/Case Studies/Dialysis
12
 Credits

RESOURCES
https://www.sciencedirect.com/topics/engin
eering/semipermeable-membrane

https://chem.libretexts.org/Bookshelve
s/Physical
and Theoretical Chemistry Textbook
Maps/Supplemental Modules (Physical
and Theoretical Chemistry)/Physical
Properties of Matter/Solutions and
Mixtures/Case Studies/Dialysis
13
 Credits

RESOURCES
https://www.sciencedirect.com/topics/engin
eering/semipermeable-membrane

14