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    A research team from the Center of Excellence for Genomic Medical Research at the Innovation Center for Personalized Medicine has won a grant. The King Abdulaziz City for Science and Technology announced the establishment of three technical innovation centers with a total budget of more than 150 million riyals. The research team's proposal from the Center of Excellence for Genomic Medical Research was one of three proposals that won the program's grant out of more than 100 proposals submitted from various universities in the Kingdom.

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    Basics of tics

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    A research team from the Center of Excellence for Genomic Medical Research at the Innovation Center for Personalized Medicine has won a grant. The King Abdulaziz City for Science and Technology announced the establishment of three technical innovation centers with a total budget of more than 150 million riyals. The research team's proposal from the Center of Excellence for Genomic Medical Research was one of three proposals that won the program's grant out of more than 100 proposals submitted from various universities in the Kingdom.

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    19 views23 pages

    Basics of Tics

    Uploaded by

    lethy2000
    A research team from the Center of Excellence for Genomic Medical Research at the Innovation Center for Personalized Medicine has won a grant. The King Abdulaziz City for Science and Technology announced the establishment of three technical innovation centers with a total budget of more than 150 million riyals. The research team's proposal from the Center of Excellence for Genomic Medical Research was one of three proposals that won the program's grant out of more than 100 proposals submitted from various universities in the Kingdom.

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    Attribution Non-Commercial (BY-NC)
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    of 23

    Basics of Bioinformatics

    Dr: A. Ellethy

    College of Dentistry

    Lec. 2

    1 4 3 1
    Top secretes to study DNA sequences
    • What, why, when, where study gene?
    • How to design a primer?
    • Types of practical used primers
    • How to read DNA sequences (the most recently
    developed program in USA).
    • Data analysis for sequenced DNA
    What,
    why,
    when,
    where
    study
    gene?
    WHERE IS DNA? WHY?
    Under sterile conditions

    Automated DNA sequencer


    H2O
    To amplify DNA sequences? 12 ul
    5X Q soln
    5
    10X buffer
    2.5
    50X dNTPs
    0.5
    primer A
    1
    primer B
    1
    Labeled ddNTPs with

    Radioisotopes S35

    Fluorescently labeled
    PCR PROGRAME

    1 cycle: 97°C 2 min

    30 cycles:
    Denaturation 97°C 1 min
    Annealing X°C 1 min’
    Extension 72°C 1min

    1 cycle:
    72°C 6 min

    store:
    4°C store

    X based melting temperature: 55-60 °C


    HOW TO READ RADIOGRAPHIC SHEETS OF DNA
    SEQUENCES?
    2 hours run 1
    AGCT

    4 hours run 2

    Start to read DNA sequences


    AGCT
    TGCTTATTACTGCAGTCACC
    ATC

    -------------------?
    How
    seq aut
    uen om
    ces ate
    ? d
    seq
    u en
    c er
    wo
    rk
    for
    DN
    A
    DNA CHROMAS

    Open and save sequences in Applied Biosystems, Staden Chromatogram (SCF), FASTA,
    EMBL, GenBank, SwissProt, GenPept, GCG RSF and plain text formats.
    HOW TO USE DNA CHROMAS?
    Cell Chromosome DNA
    DNA SEQUENCE

    ANALYSIS

     IDENTIFICATION

     CONFIRMATION

     APPLICATION

     DIAGNOSIS

     VACCINATION

     REPAIRING
    Dentin Sialophosphoprotein EST

    ATGGTCTTATTAAGAAAAAACTTCTGTTGTACCATAGGTTATGCCCATATTTCCTCCACCACAACTTCAA
    ATCCTTCATCAGAAACGAAGAGCATAGCCACAGACTATGAAACCACCCCGACCACCACTATATCTAGCAC
    TGCCTCAGGATCTACGCCCACCCTGACCACCACTGCATCCAGCATTGCCTCAGACTCTAAGCCCACCCTG
    ACCACCACTGCATCCAGCACTGCCTCAGGATCTATGTCCACCCCGACCACCCCTGCATCCAGCACTGCCT
    CAGGCTCTACACCCACAACGACCACCACTGCATCCAGCACTGCCTCAGGATCTACACCTACCCCGACCAC
    TACTGCATCTAGCAAAGCCTCAAGATCTGTGCCCACCACTGTATCCAGCACTGGCTCAGGCTCTACACCC
    ACTCCGACCACCACTGCATCCAGCACTGCCTCAGGATCTACGCCCACCCTGACCACCACTCCATCCAGCA
    CTGCCTCAGGATCTACACCTACCCCGACCACCCCTGCATCCAGTACTGCCTCAAGCTCTTCACCCACCCC
    AACCACCCCTGTATCCAGCACTGCCTCAGGATCTACACCCACCCCGACCACCACTGCATCTAGCAAGGCC
    TCAGGATCTGTGCCCACCACTGTATCCAGCACTGGCTCAGGCTCTACACCCACTCTGACCACCACTGTAT
    CCAGCACTGTCTCAGACTCTACACCCACCCCGACCACCACTGCATCCAGCACTGCCTCAGGATCAGCACC
    CACCCCGACCACCACTGCATCTAGCACTGCCTCAGGATCTATGCCCACCCTGACCACCAATGCATCCAGC
    Put original DNA sequences

    Put original test sequences


    Blastn dentin sequences
    ‫فوز الفريق البحثي لمركز التميز لبحوث الجينوم الطبية بمركز ابتكار في المجال‬
    ‫الطبي الشخصي (‪)1431-1430‬‬
    ‫‪     ‬أعلن;ت مدين;ة المل;ك عب;د العزي;ز للعلوم والتقني;ة ع;ن إنشاء ثالث;ة مراك;ز ابتك;ار تقن;ي بتكلفة‬
    ‫إجمالي;ة قدره;ا اكث;ر م;ن ‪ 150‬مليون لاير أ;ي بم;ا يعادل ‪ 50‬ميلون لاير لك;ل مركز ابتكار ‪،‬‬
    ‫وذلك ضم;ن برنام;ج م;راك;ز اإلبتك;ار التقني احد برام;ج الخطة الوطنية للعلوم والتقنية واإلبتكار ‪.‬‬
    ‫وق;د ت;م إختيار المقترح الم;قدم م;ن الفري;ق البحث;ي بمرك;ز التمي;ز لبحوث الجينوم الطبية ليكون‬
    ‫ضم;ن الثالث مقترحات الت;ي فازت بم;ن;ح ال;برنامج م;ن بي;ن أك;ث;ر م;ن ‪ 100‬مقترح مقدم من‬
    ‫جامع;ات الم;ملكة المختلفة ‪.‬‬

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