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Author: Wilsie M. Salas M.D
Author: Wilsie M. Salas M.D
infections
Principle:
0 20 0
1 45 45
2 25 50
3 5 15
4 5 20
◦ Subjective method
◦ Two slides be read by 2 different clinical laboratory
technologist
◦ The scores done by the the 2 technologist should
agree by 10%
◦ If nota 3rd should be obtained
◦ Eosinophils should be identified from the
neutrophils
◦ Laboratory values should be established by each
laboratory
LEUKOCYTE ALKALINE PHOSPHATASE (LAP)
Interpretation of LAP Score
◦ Normal 20-100
◦ Untreated Chronic Myelogeous Leukemia: decrease
◦ Leukemoid reaction: high normal to increase
◦ Low LAP Scores:
Paroxysmal Nocturnal Hemoglobinuria
Sideroblatic Anemia
Myelodysplastic Disorders
◦ High LAP Scores:
Pregnancy in the third trimester
Polycythemia Vera
FINDING SCORE
Normal 20-100
Chronic Myelogenous Leukemia <13
Leukemoid reaction >100
Polycythemia Vera 100-200
Secondary Polycythemia 20-100
ACID PHOSPHATASE (Tartrate Resistant)
Detection of Hairy Cell Leukemia
All cells contain 7 non-erythroid isoenzymes:
Isoensymes: 0, 1, 2, 3, 3b, 4 and 5
Hairy cells is isoenzyme 5 positive
Principle:
Lymphocyte + -
Hairy Cells + +